Fischer S J, Podratz J L, Windebank A J
Molecular Neuroscience Program, Mayo Graduate and Medical Schools, Rochester, MN 55905, USA.
Neurosci Lett. 2001 Jul 27;308(1):1-4. doi: 10.1016/s0304-3940(01)01956-5.
Nerve growth factor (NGF) rescues dorsal root ganglion neurons and PC12 cells from cisplatin-induced cell death. Two model systems were used to demonstrate that rescue is mediated through the high affinity NGF receptor. In dorsal root ganglion (DRG) neurons isolated from p75(-/-) and control mice, 20 ng/ml NGF completely prevented cisplatin-induced death. In PC12 cells, we overexpressed receptor chimeras between the tumor necrosis factor and NGF receptors. We demonstrated that activation of the intracellular domain of Trk A is responsible for the NGF rescue effect.
神经生长因子（NGF）可挽救背根神经节神经元和PC12细胞免受顺铂诱导的细胞死亡。使用了两个模型系统来证明这种挽救作用是通过高亲和力NGF受体介导的。在从p75基因敲除小鼠和对照小鼠分离出的背根神经节（DRG）神经元中，20 ng/ml的NGF完全阻止了顺铂诱导的死亡。在PC12细胞中，我们过表达了肿瘤坏死因子受体和NGF受体之间的受体嵌合体。我们证明，Trk A细胞内结构域的激活是NGF挽救作用的原因。
