Bliss S K, Gavrilescu L C, Alcaraz A, Denkers E Y
Departments of Microbiology and Immunology, College of Veterinary Medicine, Cornell University, Ithaca, New York 14853-6401, USA.
Infect Immun. 2001 Aug;69(8):4898-905. doi: 10.1128/IAI.69.8.4898-4905.2001.
The immunomodulatory role of neutrophils during infection with Toxoplasma gondii was investigated. Monoclonal antibody-mediated depletion revealed that neutrophils are essential for survival during the first few days of infection. Moreover, neutrophil depletion was associated with a weaker type 1 immune response as measured by decreased levels of gamma interferon, interleukin-12 (IL-12) and tumor necrosis factor alpha. IL-10 was also decreased in depleted animals. Additionally, splenic populations of CD4(+) T cells, CD8(+) T cells, and NK1.1(+) cells were decreased in depleted mice. Neutrophil-depleted mice exhibited lesions of greater severity in tissues examined and a greater parasite burden as determined by histopathology and reverse transcription-PCR. We conclude that neutrophils are critical near the time of infection because they influence the character of the immune response and control tachyzoite replication.
研究了中性粒细胞在刚地弓形虫感染过程中的免疫调节作用。单克隆抗体介导的清除实验表明，中性粒细胞对于感染初期的存活至关重要。此外，通过检测γ干扰素、白细胞介素-12（IL-12）和肿瘤坏死因子α水平降低发现，中性粒细胞清除与较弱的1型免疫反应相关。在清除中性粒细胞的动物中，IL-10水平也降低。此外，清除中性粒细胞的小鼠脾脏中CD4(+) T细胞、CD8(+) T细胞和NK1.1(+)细胞数量减少。通过组织病理学和逆转录-聚合酶链反应确定，清除中性粒细胞的小鼠在所检查的组织中表现出更严重的病变和更大的寄生虫负荷。我们得出结论，中性粒细胞在感染初期至关重要，因为它们影响免疫反应的特征并控制速殖子的复制。
