针对CC531大鼠结肠腺癌的新型小鼠单克隆抗体的研发。
The development of novel mouse monoclonal antibodies against the CC531 rat colon adenocarcinoma.
作者信息
Hagenaars M, Koelemij R, Ensink N G, van Eendenburg J D, van Vlierberghe R L, Eggermont A M, van de Velde C J, Fleuren G J, Kuppen P J
机构信息
Department of Surgery, Leiden University Medical Center, The Netherlands.
出版信息
Clin Exp Metastasis. 2000;18(4):281-9. doi: 10.1023/a:1011062002851.
In this paper we describe 4 new monoclonal antibodies to be applied in rat models for cancer. The monoclonal antibodies were obtained by immunizing Balb/c mice with CC531 rat colon adenocarcinoma cells. Hybridomas were produced and 4 were selected for their reactivity with CC531 in vitro (MG1, 2, 3 and 4). All 4 antibodies recognized other rat tumour cell lines and showed limited cross-reactivity with normal rat tissues. Intraperitoneally injected MG1, 2 and 4 homed to in vivo growing, artificially induced CC531 liver metastases. In these in vivo experiments, limited cross-reactivity with normal rat tissues, predominantly of the gastro-intestinal tract, was found. MG4 was found to enhance lysis of CC531 tumour cells mediated by IL-2 activated, cultured natural killer cells. These antibodies are potentially useful for antibody-based laboratory techniques and for investigation of antibody-based immunotherapy of cancer in a rat model.
在本文中，我们描述了4种新的单克隆抗体，它们将应用于大鼠癌症模型。这些单克隆抗体是通过用CC531大鼠结肠腺癌细胞免疫Balb/c小鼠获得的。产生了杂交瘤，并选择了4种在体外与CC531有反应性的杂交瘤（MG1、2、3和4）。所有4种抗体都能识别其他大鼠肿瘤细胞系，并且与正常大鼠组织的交叉反应性有限。腹腔注射的MG1、2和4归巢到体内生长的、人工诱导的CC531肝转移灶。在这些体内实验中，发现与正常大鼠组织（主要是胃肠道）的交叉反应性有限。发现MG4可增强IL-2激活的培养自然杀伤细胞介导的CC531肿瘤细胞的裂解。这些抗体可能对基于抗体的实验室技术以及在大鼠模型中研究基于抗体的癌症免疫治疗有用。
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