Thomas C, Nijenhuis A M, Timens W, Kuppen P J, Daemen T, Scherphof G L
Department of Physiological Chemistry, University of Groningen, The Netherlands.
Invasion Metastasis. 1993;13(2):102-12.
Inoculation of 3 x 10(4) to 3 x 10(5) CC531 colon adenocarcinoma cells into the portal vein of syngeneic WAG/Rij rats provides a reproducible animal model of colon cancer liver metastasis with macroscopically visible tumor nodules at day 25 after inoculation. In this study, the inflammatory cell response in the liver sinusoids against locally induced metastases was also investigated using immunohistochemical methods. Host immune reactive cells accumulated in the liver sinusoids of tumor-bearing livers, especially resident Kupffer cells (KC; ED1+/ED2+), and to a lesser extent pit cells (OX8+/0X19-), T lymphocytes (OX8+/OX19+) and polymorphonuclear leukocytes (PMN; HIS48+). All these cell types may be involved in the lysis of CC531 cells in the liver, although only major histocompatibility complex class II+ monocytes (3D11+/ED1+/ED2-), but not resident KC, T lymphocytes nor PMN, accumulated at the peritumoral area between liver parenchyma and tumor foci. Especially monocytes but also PMN, lymphocytes and pit cells appeared to infiltrate the stromal tumor compartment. In view of the low cell dose needed, the rat liver metastasis model of colon cancer used in this study reflects well the human, weakly immunogenic, tumor-host relationships and provides an excellent opportunity for the study of cellular reactions related to chemo- and/or immunotherapy protocols.
将3×10⁴至3×10⁵个CC531结肠腺癌细胞接种到同基因WAG/Rij大鼠的门静脉中，可建立一种可重复的结肠癌肝转移动物模型，接种后第25天可出现肉眼可见的肿瘤结节。在本研究中，还使用免疫组化方法研究了肝血窦对局部诱导转移的炎症细胞反应。宿主免疫反应性细胞聚集在荷瘤肝脏的肝血窦中，尤其是驻留的库普弗细胞（KC；ED1⁺/ED2⁺），以及程度较轻的肝窦内皮细胞（OX8⁺/0X19⁻）、T淋巴细胞（OX8⁺/OX19⁺）和多形核白细胞（PMN；HIS48⁺）。所有这些细胞类型可能都参与了肝脏中CC531细胞的裂解，尽管只有主要组织相容性复合体II类⁺单核细胞（3D11⁺/ED1⁺/ED2⁻），而不是驻留的KC、T淋巴细胞或PMN，聚集在肝实质和肿瘤灶之间的肿瘤周围区域。尤其是单核细胞，但PMN、淋巴细胞和肝窦内皮细胞似乎也浸润了肿瘤间质。鉴于所需的细胞剂量较低，本研究中使用的大鼠结肠癌肝转移模型很好地反映了人类弱免疫原性的肿瘤-宿主关系，并为研究与化学和/或免疫治疗方案相关的细胞反应提供了绝佳机会。
