Houng H S, Sethabutr O, Nirdnoy W, Katz D E, Pang L W
Department of Enteric Infections, Walter Reed Army Institute of Research, Silver Spring, MD 20910-7500, USA.
Diagn Microbiol Infect Dis. 2001 May-Jun;40(1-2):11-9. doi: 10.1016/s0732-8893(01)00251-6.
A novel ceuE-based multiplex PCR system was developed as an efficient diagnostics test to detect and differentiate C. jejuni and C. coli. There is no cross reactivity between C. jejuni and C. coli. In addition, the assay does not produce a positive signal from other enteric bacteria including Salmonella, Shigella and Escherichia coli strains. Campylobacter detection sensitivity was determined to be equivalent to previously reported PCR for other enteric bacteria. We also noticed that silicon dioxide extraction can improve Campylobacter detection sensitivity from infected stool samples. It was demonstrated that the PCR assay developed in this study had a much better Campylobacter detection rate than the traditional culturing method (77% versus 56%). However, we also identified small numbers of culture positive stools (8%, or 16 out of 202 samples) that did not yield PCR positive results for Campylobacter. These PCR negative/culture positive stools were proven to be inhibitory to PCR amplification.
开发了一种基于ceuE的新型多重PCR系统，作为一种高效的诊断测试，用于检测和区分空肠弯曲菌和结肠弯曲菌。空肠弯曲菌和结肠弯曲菌之间没有交叉反应。此外，该检测方法不会对包括沙门氏菌、志贺氏菌和大肠杆菌菌株在内的其他肠道细菌产生阳性信号。弯曲杆菌检测灵敏度被确定与先前报道的针对其他肠道细菌的PCR相当。我们还注意到，二氧化硅提取可以提高从受感染粪便样本中检测弯曲杆菌的灵敏度。结果表明，本研究开发的PCR检测方法的弯曲杆菌检出率比传统培养方法要好得多（77%对56%）。然而，我们也发现少量培养阳性的粪便样本（8%，即202个样本中的16个）对弯曲杆菌未产生PCR阳性结果。这些PCR阴性/培养阳性的粪便样本被证明对PCR扩增有抑制作用。
