Nakari U-M, Puhakka A, Siitonen A
National Public Health Institute, Mannerheimintie 166, 00300, Helsinki, Finland.
Eur J Clin Microbiol Infect Dis. 2008 Jul;27(7):513-8. doi: 10.1007/s10096-008-0467-9. Epub 2008 Mar 4.
Hippurate hydrolysis test results of 240 Campylobacter strains were compared with those of two multiplex polymerase chain reaction (PCR) assays. Of the 152 strains identified in Finnish clinical microbiology routine laboratories as C. coli (hippurate-negative), 11% were C. jejuni (hippurate-positive) by standardized hippurate test and 39% by PCR in the reference laboratory. Two of the 81 hippurate-positive strains were identified as C. coli. Standardizing the hippurate test by determining minimum and maximum turbidity limits (McFarland 6 and McFarland 10, OD(450) values 0.8 and 1.4, respectively) for the bacterial cell suspension eliminated the false-positive results, but 32% of the 145 hippurate-negative strains were still identified as C. jejuni by PCR. The species identification of Campylobacter isolates in Finland could be improved by using a standardized hippurate hydrolysis test to identify hippurate-positive C. jejuni and testing hippurate-negative strains by molecular methods. This would also improve the epidemiological data on this important zoonotic pathogen.
将240株弯曲杆菌菌株的马尿酸盐水解试验结果与两种多重聚合酶链反应（PCR）检测结果进行了比较。在芬兰临床微生物学常规实验室鉴定为大肠弯曲杆菌（马尿酸盐阴性）的152株菌株中，通过标准化马尿酸盐试验，11%为空肠弯曲杆菌（马尿酸盐阳性），而在参考实验室通过PCR检测，这一比例为39%。在81株马尿酸盐阳性菌株中，有2株被鉴定为大肠弯曲杆菌。通过确定细菌细胞悬液的最小和最大浊度限度（分别为麦氏6和麦氏10，OD(450)值分别为0.8和1.4）来标准化马尿酸盐试验，消除了假阳性结果，但145株马尿酸盐阴性菌株中仍有32%通过PCR被鉴定为空肠弯曲杆菌。通过使用标准化的马尿酸盐水解试验来鉴定马尿酸盐阳性的空肠弯曲杆菌，并通过分子方法检测马尿酸盐阴性菌株，可以改进芬兰弯曲杆菌分离株的菌种鉴定。这也将改善关于这种重要人畜共患病原体的流行病学数据。
