Nishie A, Masuda K, Otsubo M, Migita T, Tsuneyoshi M, Kohno K, Shuin T, Naito S, Ono M, Kuwano M
Departments of Medical Biochemistry, Graduate School of Medical Sciences, Kyushu University, Fukuoka 812-8582, Japan.
Clin Cancer Res. 2001 Jul;7(7):2145-51.
We used suppression subtractive hybridization to identify highly expressed genes in the cancerous region of human renal cell carcinoma (RCC) compared with noncancerous tissue. Nine genes were identified to show increased expression in the cancerous region compared with the noncancerous region. The nine genes included thymosin beta4, secreted protein acidic and rich in cysteine (SPARC), Cap43, ceruloplasmin, serum amyloid A, osteopontin, heat shock protein 90 (HSP90), LOT1, and casein kinase I. Of these 9 genes, in situ hybridization with 10 clinical samples consistently showed a strong expression of Cap43 mRNA in infiltrating macrophages in RCCs, but not in cancer cells proliferating in an alveolar pattern. However, Cap43 mRNA was also apparently detected in epithelial cells of the renal proximal tubuli in noncancerous tissue. The higher expression of the Cap43 gene in the cancerous region of RCCs appears to depend on macrophage infiltration. Moreover, treatment with phorbol ester resulted in enhanced expression of the Cap43 gene in human monocytic cells in vitro. The expression of the Cap43 gene in infiltrating macrophages is discussed in association with the differentiated or activated status of monocyte/macrophage.
我们采用抑制消减杂交技术，以鉴定与癌旁组织相比，人肾细胞癌（RCC）癌区中高表达的基因。与癌旁区域相比，共鉴定出9个基因在癌区表达增加。这9个基因包括胸腺素β4、富含半胱氨酸的酸性分泌蛋白（SPARC）、Cap43、铜蓝蛋白、血清淀粉样蛋白A、骨桥蛋白、热休克蛋白90（HSP90）、LOT1和酪蛋白激酶I。在这9个基因中，对10例临床样本进行原位杂交，结果始终显示Cap43 mRNA在RCC浸润性巨噬细胞中强烈表达，而在呈肺泡样增殖的癌细胞中不表达。然而，在癌旁组织的肾近端小管上皮细胞中也明显检测到Cap43 mRNA。RCC癌区Cap43基因的高表达似乎依赖于巨噬细胞浸润。此外，佛波酯处理可导致人单核细胞中Cap43基因在体外表达增强。本文结合单核细胞/巨噬细胞的分化或激活状态，对浸润性巨噬细胞中Cap43基因的表达进行了讨论。
