Vijay H M, Huang H, Young N M, Bernstein I L
Int Arch Allergy Appl Immunol. 1979;60(3):229-39. doi: 10.1159/000232347.
Extracts of Alternaria tenuis and Alternaria solani were separated into dialyzable (molecular weight less than 10,000) and non-dialyzable forms. The latter was further fractionated by gel filtration through Sephadex G-100 followed by ion-exchange chromatography on DEAE-cellulose. The dialyzable material was fractionated by gel filtration through Sephadex G-50. The allergenic activities of the fractions obtained from the A. tenuis extract was measured in vitro by the radioallergosorbent test assay and the allergenic potency was measured by radioallergosorbent test inhibition assay. Allergenic activity was detected in most of the non-dialyzable fractions, the majority of the activity being in the last G-100 fraction (MW approximately 20,000) which was predominantly protein in nature. The same component may be responsible for the activity found in the dialyzate and its first G-50 fraction since the immunodiffusion studies indicated that the last G-100 fraction has antigenic components in common with those of the first G-50 fraction. In addition, cross-reactions between A. tenuis and A. solani extracts show that the two species share common antigenic determinants.
细交链孢菌和茄链格孢菌的提取物被分离成可透析的（分子量小于10,000）和不可透析的形式。后者通过Sephadex G - 100凝胶过滤进一步分级，随后在DEAE - 纤维素上进行离子交换色谱。可透析物质通过Sephadex G - 50凝胶过滤进行分级。从细交链孢菌提取物获得的各部分的变应原活性通过放射变应原吸附试验测定，变应原效力通过放射变应原吸附试验抑制测定进行测量。在大多数不可透析部分中检测到变应原活性，大部分活性存在于最后一个G - 100部分（分子量约20,000），其本质上主要是蛋白质。由于免疫扩散研究表明最后一个G - 100部分与第一个G - 50部分具有共同的抗原成分，所以相同的成分可能是透析液及其第一个G - 50部分中发现的活性的原因。此外，细交链孢菌和茄链格孢菌提取物之间的交叉反应表明这两个物种共享共同的抗原决定簇。
