Yunginger J W, Roberts G D, Gleich G J
J Allergy Clin Immunol. 1976 Apr;57(4):293-301. doi: 10.1016/0091-6749(76)90085-3.
The ability to covalently couple Alternaria allergens to microcrystalline cellulose particles has permitted not only the measurement of IgE antibodies to Alternaria in patient serums but also the identification of allergenic fractions from crude Alternaria extracts. Crude aqueous Alternaria extracts from 3 commerical suppliers were coupled to cellulose but failed to bind more than 5% of total radioactive counts (TRC) when reacted with serums from highly sensitive patients. Fractionation of a commercial extract through Sephadex G-25 showed that almost all allergenic activity was located in a protein- and carbohydrate-containing peak eluting at the column void volume. These fractions were pooled and coupled to cellulose to yield a RAST polymer which produced up to 20% TRC binding when tested with serums from over 100 Alternaria-sensitive patients, and only up to 1% TRC binding with 17 nonallergic serums. The study of commercial Alternaria extracts by chromatographic and Rast inhibition techniques showed that present extracts are neither qualitatively or quantitatively comparable.
将链格孢属过敏原与微晶纤维素颗粒进行共价偶联的能力，不仅使得在患者血清中检测针对链格孢属的IgE抗体成为可能，还能从链格孢属粗提物中鉴定出变应原组分。来自3个商业供应商的链格孢属粗水提物与纤维素偶联，但在与高敏患者的血清反应时，结合的总放射性计数（TRC）不超过5%。通过葡聚糖G-25对一种商业提取物进行分级分离显示，几乎所有的变应原活性都位于在柱空体积处洗脱的一个含蛋白质和碳水化合物的峰中。将这些组分合并并与纤维素偶联，得到一种放射性变应原吸附试验（RAST）聚合物，在用100多名对链格孢属过敏患者的血清进行检测时，其产生的TRC结合率高达20%，而与17份非过敏血清反应时，TRC结合率仅高达1%。通过色谱和RAST抑制技术对商业链格孢属提取物进行研究表明，目前的提取物在质量或数量上均无可比性。
