Benítez D, García-Ortega P, Picado C, Milà J, Vives J, Martínez J, Vilella R
Servei Immunologia, IDIBAPS, Hospital Clínic Barcelona.
Allergol Immunopathol (Madr). 2001 Jan-Feb;29(1):9-15. doi: 10.1016/s0301-0546(01)79009-x.
Phleum pratense (Phl p) pollen is a known cause of allergic disease worldwide. Profilins have been identified as functional plant pan-allergens. The role of Phl p profilin in the specific immune response in sensitized Phl p patients is unknown.
Skin prick test and specific serum IgE levels were performed in 26 patients allergic to Phl p and in 18 nonallergic control donors. Peripheral blood mononuclear cells were isolated from both groups and stimulated with crude extract or highly purified Phl p profilin, and the production of type I and type II cytokines was determined in patients and controls stimulated with specific and polyclonal stimulus. T-cell lines specific to Phl p profilin were established from PBMCs and cross-reactivity with another highly purified profilin from Parietaria judaica (Pj) was evaluated.
Patients allergic to Phl p profilin showed increased T-cell-proliferative responses to this profilin compared with control subjects. The production of IL-4 and IFN-gamma in response to the specific stimulus was undetectable. However, the production of IL-4 and IFN-gamma in response to a polyclonal stimulus (PHA) was measurable and different for atopic patients and control subjects: IL-4 was higher (p < 0.001) in allergic patients and IFN-gamma lower (although not significant) in controls. Neither the T-cell responses nor the production of IL-4 in response to a polyclonal stimulus (PHA) correlated with the individual degree of cutaneous response to Phl p profilin or to the levels of specific Phl p IgE. The T-cell lines tested did not show any cross-reactivity with Pj profilin.
Phl p profilin is in part responsible for the T-cell mediated immunological response in patients allergic to Phl p. The response is very specific since Phl p profilin specific T-cell lines did not show cross-reactivity with a highly homologous profilin from Parietaria judaica (Pj). The lack of correlation between the proliferative T-cell response and polyclonal IL-4 production with allergen-specific serum IgE and SPT probably indicates that some of the responding T-cells may be involved in immune reactions other than the support of IgE production.
草地早熟禾（Phl p）花粉是全球已知的过敏性疾病病因。肌动蛋白结合蛋白已被鉴定为功能性植物泛过敏原。Phl p肌动蛋白结合蛋白在致敏的Phl p患者特异性免疫反应中的作用尚不清楚。
对26例对Phl p过敏的患者和18例非过敏对照者进行皮肤点刺试验和特异性血清IgE水平检测。从两组中分离外周血单个核细胞，用粗提物或高度纯化的Phl p肌动蛋白结合蛋白刺激，在接受特异性和多克隆刺激的患者和对照中测定I型和II型细胞因子的产生。从外周血单个核细胞建立对Phl p肌动蛋白结合蛋白特异的T细胞系，并评估其与来自墙草（Pj）的另一种高度纯化的肌动蛋白结合蛋白的交叉反应性。
与对照受试者相比，对Phl p肌动蛋白结合蛋白过敏的患者对该肌动蛋白结合蛋白的T细胞增殖反应增强。对特异性刺激的IL-4和IFN-γ产生未检测到。然而，对多克隆刺激（PHA）的IL-4和IFN-γ产生是可测量的，且特应性患者和对照受试者不同：过敏患者中IL-4较高（p < 0.001），对照中IFN-γ较低（尽管不显著）。对多克隆刺激（PHA）的T细胞反应和IL-4产生均与对Phl p肌动蛋白结合蛋白的皮肤反应个体程度或特异性Phl p IgE水平无关。所测试的T细胞系未显示与Pj肌动蛋白结合蛋白有任何交叉反应性。
Phl p肌动蛋白结合蛋白部分负责对Phl p过敏患者的T细胞介导的免疫反应。该反应非常特异，因为Phl p肌动蛋白结合蛋白特异的T细胞系未显示与来自墙草（Pj）的高度同源的肌动蛋白结合蛋白有交叉反应性。增殖性T细胞反应和多克隆IL-4产生与过敏原特异性血清IgE和皮肤点刺试验之间缺乏相关性，可能表明一些反应性T细胞可能参与了除支持IgE产生之外的免疫反应。
