Benítez D, García-Ortega P, Picado C, Milà J, Vives J, Martínez J, Vilella R
Service of Immundogy. IDIBAPS. Hospital Clínic, Barcelona. Spain.
Allergol Immunopathol (Madr). 2002 Mar-Apr;30(2):62-9. doi: 10.1016/s0301-0546(02)79092-7.
allergic disease caused by Parietaria judaica (Pj) has been widely documented in Mediterranean area. Profilins have been identified as widely distributed allergenic proteins. The role of Pj profilin in specific immune response in Pj-sensitized patients is unknown.
skin prick test and determination of specific and total IgE levels in serum were performed in all patients (n = 28) and non-allergic controls (n = 18). Peripheral blood mononuclear cells (PBMC) were isolated from both groups and stimulated with crude extract or highly purified Pj profilin. The production of type I and type II cytokines was determined by specific and polyclonal stimuli in patients and controls. T-cell lines specific to Pj profilin were established and cross-reactivity with another highly purified profilin from Phleum pratense (Phl p) was evaluated.
Pj profilin-sensitized patients showed a small but significantly increased in T-cell proliferative response to this profilin compared with non-atopic controls. The production of interleukin (IL)-4 and interferon (IFN)-γ in response to the specific stimulus was undetectable. However, the production of IL-4 in response to a polyclonal stimulus [phytohemagglutinin (PHA)] was significantly higher in atopic patients than in controls. The T-cell response did not correlate with the magnitude of response to skin prick tests with Pj profilin or with Pj-specific serum IgE levels. In addition, the production of IL-4 in response to a polyclonal stimulus (PHA) did not correlate with the individual skin prick tests to Pj profilin or with Pj-specific IgE levels in serum. The T-cell lines tested showed no cross-reactivity with Phl p profilin.
our results suggest that Pj profilin is partly responsible for the T-cell-mediated response in patients allergic to Pj. The high skin reactivity to Pj profilin is these patients was accompanied by a small increase in the T-cell response to this profilin. The response was highly specific since Pj profilin specific T-cell lines showed no cross-reactivity with a highly homologous profilin from Phl p. The lack of correlation between the proliferative T-cell response and polyclonal IL-4 production with allergen-specific serum IgE and skin reactivity probably indicates that some of the responding T-cells may be involved in immune reactions other than those supporting IgE production.
在地中海地区，由墙草（Pj）引起的过敏性疾病已有广泛记载。肌动蛋白已被确定为广泛分布的致敏蛋白。Pj肌动蛋白在Pj致敏患者的特异性免疫反应中的作用尚不清楚。
对所有患者（n = 28）和非过敏性对照者（n = 18）进行皮肤点刺试验以及血清中特异性IgE和总IgE水平的测定。从两组中分离外周血单个核细胞（PBMC），并用粗提物或高度纯化的Pj肌动蛋白进行刺激。通过对患者和对照者的特异性和多克隆刺激来测定I型和II型细胞因子的产生。建立针对Pj肌动蛋白的T细胞系，并评估其与来自梯牧草（Phl p）的另一种高度纯化的肌动蛋白的交叉反应性。
与非特应性对照者相比，Pj肌动蛋白致敏患者对该肌动蛋白的T细胞增殖反应虽小但显著增加。对特异性刺激的白细胞介素（IL）-4和干扰素（IFN）-γ产生未检测到。然而，特应性患者对多克隆刺激[植物血凝素（PHA）]的IL-4产生显著高于对照者。T细胞反应与对Pj肌动蛋白皮肤点刺试验的反应程度或Pj特异性血清IgE水平无关。此外，对多克隆刺激（PHA）的IL-4产生与对Pj肌动蛋白的个体皮肤点刺试验或血清中Pj特异性IgE水平无关。所测试的T细胞系与Phl p肌动蛋白无交叉反应。
我们的结果表明，Pj肌动蛋白在对Pj过敏的患者的T细胞介导反应中起部分作用。这些患者对Pj肌动蛋白的高皮肤反应性伴随着对该肌动蛋白的T细胞反应的小幅增加。该反应具有高度特异性，因为Pj肌动蛋白特异性T细胞系与来自Phl p的高度同源肌动蛋白无交叉反应。增殖性T细胞反应与多克隆IL-4产生以及过敏原特异性血清IgE和皮肤反应性之间缺乏相关性，这可能表明一些反应性T细胞可能参与了除支持IgE产生之外的免疫反应。
