Bauerfeind R, Barth S, Weiss R, Baljer G
Institut für Hygiene und Infektionskrankheiten der Tiere der Justus-Liebig-Universität Giessen.
Dtsch Tierarztl Wochenschr. 2001 Jun;108(6):243-5.
Strains of Salmonella isolated from animals in Germany (n = 878) were analysed for the presence of the spvD gene ("Salmonella plasmid virulence gene D") by DNA-DNA hybridization. The spvD gene was only detected in strains of serovars Typhimurium (93.3%), Enteritidis (97.1%), and Dublin (100%) as well as in two rough strains of Salmonella enterica. Salmonella isolates from mammals carried the gene more frequently (cattle 94.0%, horses 92.6%, pigs 73.7%) than those from birds (33.3%) or reptiles (4.5%). Due to its high prevalence in epidemiologically relevant salmonellae, the virulence factor spvD may represent a sensitive and specific target in various serovars for diagnostic or immunization strategies.
通过DNA-DNA杂交分析了从德国动物中分离出的878株沙门氏菌,以检测spvD基因(“沙门氏菌质粒毒力基因D”)的存在情况。spvD基因仅在鼠伤寒血清型(93.3%)、肠炎血清型(97.1%)和都柏林血清型(100%)菌株以及两株肠炎沙门氏菌粗糙型菌株中检测到。来自哺乳动物的沙门氏菌分离株携带该基因的频率(牛94.0%,马92.6%,猪73.7%)高于来自鸟类(33.3%)或爬行动物(4.5%)的分离株。由于其在具有流行病学相关性的沙门氏菌中普遍存在,毒力因子spvD可能是各种血清型诊断或免疫策略中的一个敏感且特异的靶点。