Naruse H, Tanaka K, Kim A
Department of Neurosurgery, Higashisumiyoshi Morimoto Hospital, Osaka City, Japan.
Acta Neurochir Suppl. 2000;76:101-4. doi: 10.1007/978-3-7091-6346-7_21.
The experimental spinal cord edema was produced in a cat by the infusion method of Marmarou. Horseradish peroxidase (HRP) dissolved in autoserum of a cat was used as a tracer. After laminectomy, a 30-gauge needle was inserted into the intumescentia cervicalis. A total amount of 20 microliters of a tracer was infused at a rate of 10 microliters/hr. The structural features were studied immediately and 3 days after infusion. Immediately after infusion, HRP was noted in the infused white and gray matters. Though the perivascular space in the white matter at the infused site was widely distended and filled with HRP, the space in the gray matter was not distended but filled with HRP. HRP which was observed along vessels led to the surface of the spinal cord. Swelling of astrocyte was not observed. Three days after infusion, the extracellular space and the perivascular space in the infused white matter were still expanded but were not filled with HRP. The fine structural features were similar to the findings as seen in Marmarou's infusion type of brain edema. Using this model, it seems to be feasible to study the resolution process of spinal cord edema.
采用马尔马罗（Marmarou）的输注方法在猫身上制造实验性脊髓水肿。将溶解于猫自身血清中的辣根过氧化物酶（HRP）用作示踪剂。椎板切除术后，将一根30号针头插入颈膨大处。以10微升/小时的速度总共输注20微升示踪剂。在输注后立即和3天后研究结构特征。输注后立即观察到，在输注的白质和灰质中存在HRP。虽然输注部位白质中的血管周围间隙广泛扩张并充满了HRP，但灰质中的间隙未扩张但充满了HRP。沿血管观察到的HRP延伸至脊髓表面。未观察到星形胶质细胞肿胀。输注3天后，输注白质中的细胞外间隙和血管周围间隙仍然扩大，但未充满HRP。精细结构特征与马尔马罗输注型脑水肿中的发现相似。利用该模型，研究脊髓水肿的消退过程似乎是可行的。
