一种用于（±）-莫达非尼的对映选择性测定法。

An enantioselective assay for (+/-)-modafinil.

作者信息

Cass Q B, Kohn C K, Calafatti S A, Aboul-Enein H Y

机构信息

Departamento de Química, Universidade Federal de S. Carlos, Cx. Postal 676, São Carlos, SP, Brazil.

出版信息

J Pharm Biomed Anal. 2001 Aug;26(1):123-30. doi: 10.1016/s0731-7085(01)00400-9.

DOI:10.1016/s0731-7085(01)00400-9

PMID:11451649

Abstract

Enantiomers of modafinil, (diphenylmethyl)sulphinyl-2-acetamide, were separated by an amylose tris[(S)-1-phenylethylcarbamate] chiral stationary phase, using acetonitrile: water (25:75 v/v) as mobile phase, with excellent selectivity (alpha=1.4) and resolution factors (R(s)=1.5). The assay involved a solid-phase extraction of the enantiomers of modafinil from plasma using a C(18) Cartridge. A good linear relationship was obtained in the concentration of 0.15-3 microg ml(-1) for each enantiomer. The method developed is sufficiently accurate and precise to be used for clinical samples and has a good selectivity with the two main circulating metabolites: the (diphenylmethyl)sulphinyl-2-acetic acid and (diphenylmethyl)sulphonyl-2-acetamide. The use of a polysaccharide-based column on multimodal elution was explored in developing the method.

摘要

莫达非尼的对映体，即（二苯甲基）亚磺酰基-2-乙酰胺，采用直链淀粉三[（S）-1-苯乙基氨基甲酸酯]手性固定相进行分离，以乙腈：水（25:75 v/v）作为流动相，具有出色的选择性（α=1.4）和分离度因子（R(s)=1.5）。该分析方法包括使用C(18) 柱从血浆中对莫达非尼对映体进行固相萃取。每种对映体在0.15 - 3 μg ml(-1) 的浓度范围内均获得了良好的线性关系。所开发的方法足够准确和精密，可用于临床样本，并且对两种主要循环代谢物：（二苯甲基）亚磺酰基-2-乙酸和（二苯甲基）磺酰基-2-乙酰胺具有良好的选择性。在开发该方法过程中探索了基于多糖的柱在多模式洗脱中的应用。