Moachon G, Matinier D
Centre de Recherches du Laboratoire L. Lafon, Maisons-Alfort, France.
J Chromatogr B Biomed Appl. 1994 Mar 18;654(1):91-6. doi: 10.1016/0378-4347(93)e0452-v.
A sensitive and selective high-performance liquid chromatographic (HPLC) method for the simultaneous quantitation of modafinil and its acid metabolite in human plasma has been developed. The method is based on a liquid-liquid extraction followed by isocratic reversed-phase HPLC with ultraviolet absorbance detection at 236 nm. The eluent used was acetonitrile-water-acetic acid (150:420:12, v/v/v). The run time was 45 in. The method provided a detection limit of 0.04 mg/l for modafinil and the acid metabolite, a quantitation limit of 0.13 mg/l for modafinil and 0.14 mg/l for the acid metabolite. A good linear relationship was obtained in the concentration range studied (0.1-20 mg/l) for both compounds and the method was sufficiently accurate and precise to support clinical pharmacokinetic studies. To our knowledge this is the first described method for determination of modafinil and its acid metabolite in plasma.
已开发出一种灵敏且具选择性的高效液相色谱（HPLC）方法，用于同时定量测定人体血浆中的莫达非尼及其酸性代谢物。该方法基于液液萃取，随后进行等度反相HPLC，并在236 nm处采用紫外吸光度检测。所用洗脱液为乙腈 - 水 - 乙酸（150:420:12，v/v/v）。运行时间为45分钟。该方法对莫达非尼及其酸性代谢物的检测限为0.04 mg/l，莫达非尼的定量限为0.13 mg/l，酸性代谢物的定量限为0.14 mg/l。在所研究的浓度范围（0.1 - 20 mg/l）内，两种化合物均呈现良好的线性关系，且该方法足够准确和精密，可支持临床药代动力学研究。据我们所知，这是首次描述的测定血浆中莫达非尼及其酸性代谢物的方法。
