Richardson M, Ramshaw J A, Boulter D
Department of Botany, University of Durham, Durham City, Great Britain.
Biochim Biophys Acta. 1971 Dec 28;251(3):331-3. doi: 10.1016/0005-2795(71)90119-x.
The amino acid sequence of rape (Brassica Napus L) cytochrome c was determined using 1 mumole of protein. Analysis of chymotryptic and tryptic peptides by the dansyl-Edman method showed that the molecule consisted of 111 residues and was homologous with other mitochondrial plant cytochromes c. The amino acid sequence was found to be identical with that previously reported for the cytochrome c from cauliflower (Brassica oleracea L).
利用1微摩尔蛋白质测定了油菜(甘蓝型油菜)细胞色素c的氨基酸序列。通过丹磺酰-埃德曼法对胰凝乳蛋白酶和胰蛋白酶肽段进行分析表明,该分子由111个残基组成,并且与其他线粒体植物细胞色素c具有同源性。发现该氨基酸序列与先前报道的来自花椰菜(甘蓝)的细胞色素c的序列相同。