Brown R H, Boulter D
Biochem J. 1973 Jun;133(2):251-4. doi: 10.1042/bj1330251.
The amino acid sequence of cytochrome c from Nigella damascena L. was determined on 0.2mumol of protein. Peptides from a single chymotryptic digest were analysed by the dansyl-Edman procedure. These peptides were ordered by reference to the sequences of other plant cytochromes c, assuming that the Nigella cytochrome is homologous with the other cytochromes. Many of the Nigella peptides were one or two residues short when compared with the corresponding chymotryptic peptides from other plant cytochromes c. These residues are assumed to have been removed by an endogenous carboxypeptidase, and the identification and placing of these residues is entirely based on homology. These residues are numbered 3, 18, 42, 43, 44, 54, 67, 72, 73, 82 and 105. A number of other positions are almost entirely placed by homology. These are positions which could not be placed definitely by dansyl-Edman analysis or by dansylation after digestion with carboxypeptidase A, and are numbered 14, 15, 16, 39, 40, 85, 86, 87 and 88. Except for residue 15, all residues based entirely, or nearly so, on homology have been previously found invariant in sequences of plant cytochromes c. Experimental details are given in a supplementary paper that has been deposited as Supplementary Publication SUP 50017, at the National Lending Library for Science and Technology, Boston Spa, Yorks. LS23 7BQ, U.K., from whom copies can be obtained on the terms indicated in Biochem. J. (1973) 131, 5.
在0.2微摩尔蛋白质上测定了黑种草细胞色素c的氨基酸序列。来自单一胰凝乳蛋白酶消化产物的肽段通过丹磺酰-埃德曼法进行分析。参照其他植物细胞色素c的序列对这些肽段进行排序，假定黑种草细胞色素与其他细胞色素具有同源性。与其他植物细胞色素c相应的胰凝乳蛋白酶肽段相比，许多黑种草肽段短一两个残基。假定这些残基已被内源性羧肽酶去除，这些残基的鉴定和定位完全基于同源性。这些残基编号为3、18、42、43、44、54、67、72、73、82和105。其他一些位置几乎完全通过同源性定位。这些位置无法通过丹磺酰-埃德曼分析或用羧肽酶A消化后进行丹磺酰化来明确定位，编号为14、15、16、39、40、85、86、87和88。除了残基15外，所有完全或几乎完全基于同源性的残基先前在植物细胞色素c的序列中都被发现是不变的。实验细节见一篇补充论文，该论文已作为补充出版物SUP 50017存放在英国约克郡波士顿温泉市国家科技出借图书馆，邮编LS23 7BQ，可按《生物化学杂志》（1973年）131卷5期所示条件从该处获取复印件。
