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Lactoperoxidase was extracted from cow milk by a simplified method starting with batch-wise adsorption onto GM-Sephadex-50. It was then purified by (NH4)2SO4 precipitation and isoelectric focusing. This product had an A412 nm/A280 nm ratio of 0.8-0.9. 2. Lactoperoxidase together with H2O2 could oxidize carrier-free Na125I to "active iodine" with efficiency to iodinate proteins to high specific activity. 3. Polypeptide hormones radioiodinated by this technique retained their immunological reactivity and were used in radioimmunoassays with good results.

采用一种简化方法从牛奶中提取乳过氧化物酶，该方法首先将其分批吸附到GM - Sephadex - 50上，然后通过硫酸铵沉淀和等电聚焦进行纯化。该产品在412nm波长处的吸光度与280nm波长处的吸光度之比为0.8 - 0.9。2. 乳过氧化物酶与过氧化氢一起可将无载体的碘化钠（Na125I）氧化为“活性碘”，从而有效地将蛋白质碘化至高比活度。3. 用该技术进行放射性碘化的多肽激素保留了它们的免疫反应性，并用于放射免疫分析，效果良好。

Lactoperoxidase was extracted from cow milk by a simplified method starting with batch-wise adsorption onto GM-Sephadex-50. It was then purified by (NH4)2SO4 precipitation and isoelectric focusing. This product had an A412 nm/A280 nm ratio of 0.8-0.9. 2. Lactoperoxidase together with H2O2 could oxidize carrier-free Na125I to "active iodine" with efficiency to iodinate proteins to high specific activity. 3. Polypeptide hormones radioiodinated by this technique retained their immunological reactivity and were used in radioimmunoassays with good results.

采用一种简化方法从牛奶中提取乳过氧化物酶，该方法首先将其分批吸附到GM - Sephadex - 50上，然后通过硫酸铵沉淀和等电聚焦进行纯化。该产品在412nm波长处的吸光度与280nm波长处的吸光度之比为0.8 - 0.9。2. 乳过氧化物酶与过氧化氢一起可将无载体的碘化钠（Na125I）氧化为“活性碘”，从而有效地将蛋白质碘化至高比活度。3. 用该技术进行放射性碘化的多肽激素保留了它们的免疫反应性，并用于放射免疫分析，效果良好。

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