Suh H, Han D W, Park J C, Lee D H, Lee W S, Han C D
Department of Medical Engineering and Department of Orthopedic Surgery, Yonsei University College of Medicine, 134 Shinchon-dong, Seodaemun-ku, Seoul 120-752, Korea.
Artif Organs. 2001 Jun;25(6):459-66. doi: 10.1046/j.1525-1594.2001.025006459.x.
Bone inducing agent (BIA) isolated from Saos-2 human osteosarcoma cells was added to an artificial bone substitute composed of 980 degrees C-heated carbonate apatite (CAp) and Type I atelocollagen (AtCol) extracted from bovine tail skins (88/12 in wt/wt %), and a CAp-AtCol-BIA substitute was prepared as an osteoinductive bone substitute. Rat calvaria osteoblasts treated by the isolated BIA demonstrated significantly increased alkaline phosphatase (ALP) activity after 3 days (p < 0.05). In vitro cell attachment and proliferation and ALP activity were investigated for the bone substitute combined with BIA. Osteoblasts cultured onto the surface of the CAp-AtCol-BIA substitute demonstrated remarkable morphological changes such as radial spreading, flattening, and projecting filopodia after 5 days. In comparison with the substitute without BIA, osteoblasts grown in the BIA-combined CAp-AtCol substitute expressed significantly increased proliferation and ALP activity, respectively (p < 0.05). Both the substitutes combined with and without BIA were implanted into artificial defects created in rabbit radii. After 4 weeks, the CAp-AtCol-BIA substitute implanted lesion was completely replaced by regenerated host bone in radiological observation whereas the substitute without BIA was partially resorbed. No histologic abnormalities appeared in the substitute either with or without BIA.
将从人骨肉瘤细胞Saos-2中分离出的骨诱导剂（BIA）添加到由980℃加热的碳酸磷灰石（CAp）和从牛尾皮中提取的I型去端胶原蛋白（AtCol）（重量比为88/12）组成的人工骨替代物中，制备了CAp-AtCol-BIA替代物作为具有骨诱导性的骨替代物。经分离出的BIA处理的大鼠颅骨成骨细胞在3天后碱性磷酸酶（ALP）活性显著增加（p<0.05）。对结合了BIA的骨替代物进行了体外细胞附着、增殖及ALP活性研究。在CAp-AtCol-BIA替代物表面培养的成骨细胞在5天后表现出显著的形态变化，如呈放射状铺展、变平以及伸出丝状伪足。与不含BIA的替代物相比，在结合了BIA的CAp-AtCol替代物中生长的成骨细胞分别表现出显著增加的增殖和ALP活性（p<0.05）。将结合和未结合BIA的两种替代物植入兔桡骨制造的人工缺损处。4周后，放射学观察显示，植入CAp-AtCol-BIA替代物的损伤部位完全被再生的宿主骨替代，而不含BIA的替代物则部分被吸收。无论含或不含BIA，替代物均未出现组织学异常。
