Santos M H, Valerio P, Goes A M, Leite M F, Heneine L G D, Mansur H S
Department of Dentistry, Federal University of Vales do Jequitinhonha e Mucuri, FCBS/UFVJM, mailbox 38, 39.100-000, Diamantina, Minas Gerais, Brazil.
Biomed Mater. 2007 Jun;2(2):135-41. doi: 10.1088/1748-6041/2/2/012. Epub 2007 May 3.
In this work, novel composites based on calcium phosphates (CaP)/collagen (COL) doped with Zn(+2) have been synthesized. They were characterized by SEM coupled to EDS microprobe in order to evaluate their morphology and chemical composition, respectively. The biocompatibility of these synthetic CaP/COL nanocomposites doped and undoped with Zn(+2) was investigated through osteoblast cell culture assay. Calcium phosphates were produced via aqueous precipitation routes where two different phases were obtained, hydroxyapatite (HAP) and biphasic hydroxyapatite-betatricalcium phosphate (HAPbetaTCP). In the sequence, the type-I collagen (COL) was added to the inorganic phase based on calcium phosphate and the mixture was blended until a homogenous composite was obtained. Zn(+2) aqueous solution (1.0 wt%) was used as the doping reagent. The cell viability and the alkaline phosphatase production of osteoblasts in the presence of the composites were evaluated and compared to control osteoblasts. Also, the biocompatibility of the composite was investigated through cell morphological analysis using optical microscopy of osteoblasts. All experiments were performed in triplicates (n = 3) from three different experiments. They were analyzed by variance test (ANOVA) and Bonferroni's post-test with differences statistically significant at p < 0.05. The results showed that the CaP/COL composites doped and undoped with Zn(+2) did not present alterations in cell morphology in 72 h and had similar cell viability and alkaline phosphatase activity to the control. All the tested CaP/COL composites showed adequate biological properties with the potential to be used in bone tissue replacement applications.
在这项工作中，合成了基于掺杂Zn(+2)的磷酸钙（CaP）/胶原蛋白（COL）的新型复合材料。通过扫描电子显微镜（SEM）与能谱仪（EDS）微探针联用对其进行表征，以便分别评估其形态和化学成分。通过成骨细胞培养试验研究了这些掺杂和未掺杂Zn(+2)的合成CaP/COL纳米复合材料的生物相容性。磷酸钙通过水沉淀法制备，得到了两种不同的相，即羟基磷灰石（HAP）和双相羟基磷灰石-磷酸三钙（HAPβTCP）。接着，将I型胶原蛋白（COL）添加到基于磷酸钙的无机相中，并将混合物混合直至获得均匀的复合材料。使用1.0 wt%的Zn(+2)水溶液作为掺杂剂。评估了复合材料存在时成骨细胞的细胞活力和碱性磷酸酶的产生，并与对照成骨细胞进行比较。此外，通过对成骨细胞进行光学显微镜细胞形态分析来研究复合材料的生物相容性。所有实验均来自三个不同的实验，重复进行三次（n = 3）。通过方差分析（ANOVA）和Bonferroni事后检验进行分析，差异在p < 0.05时具有统计学意义。结果表明，掺杂和未掺杂Zn(+2)的CaP/COL复合材料在72小时内细胞形态未出现改变，并且与对照相比具有相似的细胞活力和碱性磷酸酶活性。所有测试的CaP/COL复合材料均显示出足够的生物学特性，具有用于骨组织替代应用的潜力。
