Matulova M, Delort A M, Nouaille R, Gaudet G, Forano E
Laboratoire de Synthèse, Electrosynthèse et Etude de Systèmes à Intérêt Biologique, UMR 6504, Université Blaise Pascal, CNRS, Aubière, France.
Eur J Biochem. 2001 Jul;268(14):3907-15. doi: 10.1046/j.1432-1327.2001.02300.x.
1D and 2D NMR experiments were used to analyse the synthesis of various metabolites by resting cells of Fibrobacter succinogenes S85 when incubated with [1-(13)C]glucose, in both extracellular and cellular media. Besides the expected glycogen, succinate, acetate, glucose-1-P and glucose-6-P, maltodextrins and cellodextrins were detected. Maltodextrins were excreted into the external medium. They were found to have linear structures with a maximum degree of polymerization (DP) of about 6 or 7 units. Cellodextrins were located in the cells (cytoplasm and/or periplasm), and their DP was < or = 4. Both labelled (1-(13)C and 6-(13)C) and unlabelled maltodextrins and cellodextrins were detected, showing the contribution of carbohydrate cycling in F. succinogenes, including the reversal of glycolysis and the futile cycle of glycogen. The mechanisms of these oligosaccharide syntheses are discussed.
采用一维和二维核磁共振实验分析了产琥珀酸丝状杆菌S85的静息细胞在与[1-(13)C]葡萄糖一起孵育时，在细胞外和细胞培养基中各种代谢产物的合成情况。除了预期的糖原、琥珀酸盐、乙酸盐、葡萄糖-1-磷酸和葡萄糖-6-磷酸外，还检测到了麦芽糊精和纤维糊精。麦芽糊精被分泌到外部培养基中。发现它们具有线性结构，最大聚合度（DP）约为6或7个单位。纤维糊精位于细胞内（细胞质和/或周质），其DP小于或等于4。同时检测到了标记的（1-(13)C和6-(13)C）以及未标记的麦芽糊精和纤维糊精，这表明了碳水化合物循环在产琥珀酸丝状杆菌中的作用，包括糖酵解的逆转和糖原的无效循环。本文讨论了这些寡糖合成的机制。
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