Nouaille Régis, Matulova Maria, Pätoprstý Vladimír, Delort Anne-Marie, Forano Evelyne
Laboratoire de Synthèse et Etude de Systèmes à Intérêt Biologique, UMR 6504 Université Blaise Pascal-CNRS, 63177 Aubière Cdex, France.
Appl Microbiol Biotechnol. 2009 Jun;83(3):425-33. doi: 10.1007/s00253-009-1884-0. Epub 2009 Jan 31.
Extracellular culture fluid of Fibrobacter succinogenes S85 grown on glucose, cellobiose, cellulose or wheat straw was analysed by 2D-NMR spectroscopy. Cellodextrins did not accumulate in the culture medium of cells grown on cellulose or straw. Maltodextrins and maltodextrin-1P were identified in the culture medium of glucose, cellobiose and cellulose grown cells. New glucose derivatives were identified in the culture fluid under all the substrate conditions. In particular, a compound identified as cellobionic acid accumulated at high levels in the medium of F. succinogenes S85 cultures. The production of cellobionic acid (and cellobionolactone also identified) was very surprising in an anaerobic bacterium. The results suggest metabolic shifts when cells were growing on solid substrate cellulose or straw compared to soluble sugars.
利用二维核磁共振光谱对在葡萄糖、纤维二糖、纤维素或麦秸上生长的琥珀酸丝状杆菌S85的细胞外培养液进行了分析。纤维糊精不会在以纤维素或麦秸为生长底物的细胞培养基中积累。在以葡萄糖、纤维二糖和纤维素为生长底物的细胞培养基中鉴定出了麦芽糊精和麦芽糊精-1P。在所有底物条件下的培养液中都鉴定出了新的葡萄糖衍生物。特别地，一种被鉴定为纤维二糖酸的化合物在琥珀酸丝状杆菌S85培养物的培养基中大量积累。在厌氧细菌中产生纤维二糖酸（以及同样被鉴定出的纤维二糖酸内酯）是非常令人惊讶的。结果表明，与可溶性糖相比，当细胞在固体底物纤维素或麦秸上生长时会发生代谢转变。
