Process Analysis of Anaerobic Fermentation Exposure to Metal Mixtures.

Anaerobic fermentation is a cost-effective biowaste disposal approach. During fermentation, microorganisms require a trace amount of metals for optimal growth and performance. This study investigated the effects of metal mixtures on biogas properties, process stability, substrate degradation, enzyme activity, and microbial communities during anaerobic fermentation. The addition of iron (Fe), nickel (Ni), and zinc (Zn) into a copper (Cu)-stressed fermentation system resulted in higher cumulative biogas yields, ammonia nitrogen (NH-N) concentrations and coenzyme F activities. Ni and Zn addition enhanced process stability and acetate utilization. The addition of these metals also improved and brought forward the peak daily biogas yields as well as increased CH content to 88.94 and 86.58%, respectively. Adding Zn into the Cu-stressed system improved the abundance of , and , the degradation of cellulose, and the transformation of CO to CH. The bacterial and archaeal communities were responsible for the degradation of lignocelluloses and CH production during the fermentation process. This study supports the reutilization of heavy metal-contaminated biowaste and provides references for further research on heavy metals impacted anaerobic fermentation.