Turri M G, Henderson N D, DeFries J C, Flint J
Wellcome Trust Centre for Human Genetics, Oxford, OX3 7BN, United Kingdom.
Genetics. 2001 Jul;158(3):1217-26. doi: 10.1093/genetics/158.3.1217.
Bidirectional selection in rodents has been used to derive animal models of human behavior. An important question is whether selection for behavior operates on a limited number of QTL or whether the number and individual contribution of QTL varies between selection experiments. To address this question, we mapped QTL in two large F2 intercrosses (N = 815 and 821) from the four lines derived from a replicated selection experiment for open-field activity, an animal model for susceptibility to anxiety. Our analyses indicate that selection operated on the same relatively small number of loci in both crosses. Haplotype information and the direction of effect of each QTL allele were used to confirm that the QTL mapped in the two crosses lie in the same chromosomal regions, although we were unable to determine whether QTL in the two crosses represent the same genes. We conclude that the genetic architecture of the selected strains is similar and relatively simple.
啮齿动物中的双向选择已被用于构建人类行为的动物模型。一个重要的问题是，行为选择是作用于有限数量的数量性状基因座（QTL），还是QTL的数量和个体贡献在不同的选择实验中有所不同。为了解决这个问题，我们在两个大型F2杂交群体（N = 815和821）中对QTL进行了定位，这两个群体来自于一个针对旷场活动的重复选择实验所衍生的四条品系，旷场活动是一种焦虑易感性的动物模型。我们的分析表明，在两个杂交群体中，选择作用于相同数量相对较少的基因座。单倍型信息以及每个QTL等位基因的效应方向被用于确认在两个杂交群体中定位的QTL位于相同的染色体区域，尽管我们无法确定两个杂交群体中的QTL是否代表相同的基因。我们得出结论，所选品系的遗传结构相似且相对简单。
