一种测定伊红商业样品的方法。

A procedure for assaying commercial samples of eosin.

作者信息

Marshall P N, Bentley S A, Lewis S M

出版信息

Stain Technol. 1975 Mar;50(2):107-13. doi: 10.3109/10520297509117043.

DOI:10.3109/10520297509117043

Abstract

A simple, rapid and inexpensive assay method for the colored components of commercial samples of eosin is described. Samples are dissolved in dilute aqueous ammonia and absorbance measurements made at wavelengths of 492 nm, 512 nm, and 518 nm. The concentrations of fluorescein, tribomofluorescein and eosin are derived by solution of simulataneous equations in three unknowns. The validity of the method has been confirmed by recovery experiments. Mean recoveries of fluorescein, tribromofluorescein and eosin were 99.1%, 100.2% and 98.8% respectively. Seventeen commercial samples have been assayed by this procedure. Concentrations of fluorescein, tribromofluorescein and eosin ranged from 0.00%-0.82%, 4.34%-29.92% and 44.89%-82.82% respectively. The sodium concentrations of the same samples were measured by atomic absorption spectrophotometry and found to range from 0.10% to 6.13%. The presence of free halide (Br-, Cl-, or both) was demonstrated. These observations, together with color variations in the samples, indicate that in commercial products eosin is in the form of both the free acid and sodium salt. Volatile matter accounted for 1.68%-16.11% of the samples.

摘要

本文描述了一种用于检测商业伊红样品中有色成分的简单、快速且廉价的分析方法。将样品溶解于稀氨水，然后在492nm、512nm和518nm波长处进行吸光度测量。通过求解含有三个未知数的联立方程得出荧光素、三溴荧光素和伊红的浓度。该方法的有效性已通过回收率实验得到证实。荧光素、三溴荧光素和伊红的平均回收率分别为99.1%、100.2%和98.8%。已用此方法分析了17个商业样品。荧光素、三溴荧光素和伊红的浓度分别在0.00%-0.82%、4.34%-29.92%和44.89%-82.82%范围内。用原子吸收分光光度法测定了相同样品的钠浓度，发现其范围在0.10%至6.13%之间。证明了游离卤化物（Br-、Cl-或两者）的存在。这些观察结果以及样品中的颜色变化表明，在商业产品中，伊红以游离酸和钠盐两种形式存在。挥发物占样品的1.68%-16.11%。