一种标准化罗曼诺夫斯基染色剂的染色特性及稳定性
Staining properties and stability of a standardised Romanowsky stain.
作者信息
Marshall P N, Bentley S A, Lewis S M
出版信息
J Clin Pathol. 1978 Mar;31(3):280-2. doi: 10.1136/jcp.31.3.280.
An evaluation of the standardised Romanowsky stain of Marshall et al. has been made in a routine haematology laboratory. It was noted that this stain had several advantages over the May-Grünwald Giemsa stain used in most British laboratories. These advantages include ease and speed of preparation, a shorter staining time, and reproducibility of results. These results are described in detail. The stability of the stock stain solution and of the 'working' stain (stock + buffer) has been studied by, respectively, thin-layer chromatography and visible spectroscopy. No change was detected in the composition of the stock solution at ambient temperature over a period of six months. Stability was unaffected by the composition of the container (polyethylene, PyrexTM, or soda-glass) or by daylight. The 'working' solution was stable for 3 hours. Thereafter a precipitate is formed, consisting of thiazine dyes and eosin in a molar ratio of approximately 2:1.
在一个常规血液学实验室对马歇尔等人的标准化罗曼诺夫斯基染色法进行了评估。注意到这种染色法相对于大多数英国实验室使用的美-格吉姆萨染色法有几个优点。这些优点包括制备简便快捷、染色时间更短以及结果的可重复性。详细描述了这些结果。分别通过薄层色谱法和可见光谱法研究了储备染色液和“工作”染色液（储备液+缓冲液）的稳定性。在室温下，储备液的成分在六个月内未检测到变化。稳定性不受容器成分（聚乙烯、派热克斯玻璃或钠玻璃）或日光的影响。“工作”溶液在3小时内稳定。此后会形成沉淀，其由摩尔比约为2:1的噻嗪染料和曙红组成。
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