Nolte B A, Lineberger R D, Reed D W, Rumpho M E
Planta Med. 2001 Jun;67(4):376-8. doi: 10.1055/s-2001-14308.
A micro-assay has been developed to extract and rapidly quantify the anticancer alkaloid, camptothecin (CPT), from two leaf disks of Camptotheca acuminata Decaisne (Nyssaceae). This assay utilizes thin-layer chromatography in conjunction with fluorescence imaging to obtain reproducible measurements in the nanogram range. A large number of trees can be screened using this procedure to identify high producers of CPT in a relatively short period of time.
已开发出一种微量分析方法，用于从两叶喜树（蓝果树科）中提取并快速定量抗癌生物碱喜树碱（CPT）。该分析方法利用薄层色谱结合荧光成像，以获得纳克范围内的可重复测量结果。使用此方法可以在相对较短的时间内筛选大量树木，以鉴定喜树碱的高产植株。
