Liu Z, Wang Y, Zhou K, Han D, Yang X, Liu X
Planta Med. 2001 Jun;67(4):385-7. doi: 10.1055/s-2001-14321.
Based on the sequences of the mitochondrial 12S rRNA gene fragment of 17 samples from Gekkonidae, Salamandridae, Agamidae and Hynobiidae, respectively, a pair of allele-specific primers was designed for differentiating the Chinese medicinal material Gecko from its adulterants by PCR. The results of amplification with the primers indicate that amplicons from the templates of Gekko gecko were clearly revealed by agarose gel electrophoresis, whereas no evident amplicons were found from other species. The primers were employed to identify crude drug samples from different sources. Among a total of 9 samples, 3 were diagnosed as genuine Gecko. This result is consistent with morphological identification and DNA sequence analyses.
分别基于壁虎科、蝾螈科、鬣蜥科和隐鳃鲵科17个样本的线粒体12S rRNA基因片段序列，设计了一对等位基因特异性引物，用于通过聚合酶链反应（PCR）区分中药材壁虎与其伪品。引物扩增结果表明，经琼脂糖凝胶电泳可清晰显示来自蛤蚧模板的扩增子，而其他物种未发现明显的扩增子。这些引物被用于鉴定不同来源的药材样本。在总共9个样本中，有3个被诊断为正品壁虎。该结果与形态学鉴定和DNA序列分析一致。
