Su Yanyan, Ding Dandan, Yao Mengjie, Wu Lan, Dong Gangqiang, Zhang Dong, Chen Shilin, Xiang Li
Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing, 100700 China.
Amway (China) Botanical Research and Development Center, Wuxi, 214145 China.
Chin Med. 2020 Sep 29;15:103. doi: 10.1186/s13020-020-00382-2. eCollection 2020.
The dry body of the Tokay Gecko () is the source of a valuable traditional Chinese medicine, it is therefore listed as a Class II protected animal species in China. Due to increasing market demand and a declining supply of the species, a considerable number of adulterants have emerged in the market. Thus, it is necessary to establish an accurate and rapid method of identification for distinguishing from its adulterants and for separating it from highly processed products.
A total of 274 sequences were analyzed by using MEGA 5.0 software. Several specific primers were designed to amplify mini-barcode regions and identify from its counterfeits and products.
274 sequences of and 15 adulterants species were analyzed. could be distinguished from its adulterants through BLAST analysis, intra- and inter-specific distance analyses, and an NJ tree based on sequences. Two pairs of specific primers designed for this study, COISF2/COISR2 and COISF3/COISR3, amplified 200- and 133-bp fragments of the region, respectively, both of which were suitable for the identification of and its adulterants. Furthermore, COISF3/COISR3 detected in 15 batches of products.
Therefore, the specific DNA mini-barcoding method developed here may be a powerful tool for the identification of and counterfeits, and may also be used to distinguish from its highly processed by-products.
蛤蚧干燥体是一种名贵中药材的来源，因此在中国被列为国家二级保护动物。由于市场需求不断增加以及该物种供应减少，市场上出现了大量掺假品。因此，有必要建立一种准确、快速的鉴别方法，以区分蛤蚧与其掺假品，并将其与深加工产品区分开来。
使用MEGA 5.0软件对总共274条蛤蚧序列进行分析。设计了几条特异性引物来扩增微型条形码区域，并从其假冒品和产品中鉴别蛤蚧。
分析了274条蛤蚧序列以及15种掺假物种的序列。通过BLAST分析、种内和种间距离分析以及基于蛤蚧序列构建的NJ树，可以区分蛤蚧与其掺假品。本研究设计的两对特异性引物COISF2/COISR2和COISF3/COISR3分别扩增出蛤蚧区域200 bp和133 bp的片段，两者均适用于蛤蚧及其掺假品的鉴别。此外，COISF3/COISR3在15批产品中检测到了蛤蚧。
因此，本文开发的特异性DNA微型条形码方法可能是鉴别蛤蚧及其假冒品的有力工具，也可用于将蛤蚧与其深加工副产品区分开来。
