苯并[a]芘急性处理后8-氧代鸟嘌呤糖基化酶（OGG1）修复中的器官特异性差异。

Organ-specific differences in 8-oxoguanosine glycosylase (OGG1) repair following acute treatment with benzo[a]pyrene.

作者信息

Stedeford T, Cardozo-Pelaez F, Hover C, Harbison R D, Sanchez-Ramos J

机构信息

Dept. of Neurology, College of Medicine, University of South Florida, Tampa, USA.

出版信息

Res Commun Mol Pathol Pharmacol. 2001 Jul;109(1-2):73-85.

PMID:11458987

Abstract

The lung has been shown to be a target organ for the deleterious effects of Benzo[a]pyrene (B[a]P), regardless of the route of exposure. 8-hydroxy-2'-deoxyguanosine (oxo8dG) is a mutagenic lesion formed in DNA following exposure to B[a]P. The objective of this study was to determine the capacity of different organs to repair oxo8dG following intraperitoneal (i.p.) treatment with B[a]P. Male Spraque-Dawley rats were administered 20 mg/kg B[a]P i.p., 2 times/day for 5 days. A 26% decrease in the capacity to remove oxo8dG was observed in lung tissue at 72 hours and recovered 20% above control values at 120 hours. The capacity of the liver and kidney remained at baseline for all time points analyzed. A 7-fold increase in oxo8dG was observed in the lung at 72 hours. This study demonstrates that organ-specific differences exist in the capacity to remove oxo8dG and further demonstrates the susceptibility of lung tissue to the effects of B[a]P.

摘要

无论暴露途径如何，肺已被证明是苯并[a]芘（B[a]P）有害影响的靶器官。8-羟基-2'-脱氧鸟苷（oxo8dG）是暴露于B[a]P后在DNA中形成的诱变损伤。本研究的目的是确定腹腔注射（i.p.）B[a]P后不同器官修复oxo8dG的能力。对雄性Spraque-Dawley大鼠腹腔注射20 mg/kg B[a]P，每天2次，持续5天。在72小时时观察到肺组织中去除oxo8dG的能力下降了26%，在120小时时恢复到比对照值高20%。在所有分析的时间点，肝脏和肾脏的能力保持在基线水平。在72小时时，肺中oxo8dG增加了7倍。这项研究表明，在去除oxo8dG的能力方面存在器官特异性差异，并进一步证明了肺组织对B[a]P影响的易感性。