Sharma Sucheta, Dani H M
Department of Biochemistry, Panjab University, Chandigarh 160 014, India.
Indian J Exp Biol. 2002 Sep;40(9):1067-70.
In order to determine the organ specific carcinogenicity of benzo(a)pyrene (B(a)P), its metabolites, formed in vitro by incubation with the homogenates from liver, lungs, kidneys, intestine and brain of rats, were isolated by TLC and spectroscopy. B(a)P was found to be converted into a number of metabolites by different tissue homogenates. The results showed that the proximate carcinogenic metabolite, 7,8-dihydro-7,8-dihydroxy B(a)P was formed only when rat lung and kidney homogenates were incubated with B(a)P in vitro. The UV spectral analysis also confirmed the formation of this metabolite only on incubation of B(a)P with rat lung and kidney homogenates. As the proximate carcinogenic metabolite was only formed by incubating B(a)P with the homogenates from target organs, its organ specific carcinogenicity may be explained.
为了确定苯并(a)芘（B(a)P）的器官特异性致癌性，通过薄层色谱法（TLC）和光谱法分离了其在体外与大鼠肝脏、肺、肾脏、肠道和脑的匀浆孵育形成的代谢产物。发现B(a)P被不同的组织匀浆转化为多种代谢产物。结果表明，仅当大鼠肺和肾匀浆在体外与B(a)P孵育时，才形成近似致癌代谢产物7,8-二氢-7,8-二羟基B(a)P。紫外光谱分析也证实仅在B(a)P与大鼠肺和肾匀浆孵育时才形成这种代谢产物。由于仅通过将B(a)P与靶器官的匀浆孵育才形成近似致癌代谢产物，因此可以解释其器官特异性致癌性。
