Boujtita M, Boitard M, Murr N E
Laboratoire d'Analyse Isotopique et Electrochimique de Métabolismes (UPRSA CNRS N 6006), Université de Nantes, Faculté des Sciences et des Techniques 2, France.
Biosens Bioelectron. 1999 Jun 30;14(6):545-53. doi: 10.1016/s0956-5663(99)00027-5.
Most biosensors reported to date have been prepared, studied and used under laboratory conditions. The feasibility of a very great number of biosensors seems to be demonstrated and their characteristics, very often, established as corresponding to the demands of the modern analysis. The operational stability of the biosensors, according to authors, is almost always acceptable. The long term storage, with analytical quality conservation that is necessary to commercialise products, has rarely been studied. The stability of biosensors has to remain not only during the fabrication step or their subsequent utilisation, but also throughout the whole commercial shelf-life of the sensor, from producer to end user, through wholesaler and/or retailer. We developed the manufacturing processes, on a large scale, of renewable surface electrodes modified with enzymes such as oxidoreductases. The process consisting of several steps is described and the analytical behaviours of resulting biosensors is studied and correlated with the effects of different constraints applied during the fabrication process.
迄今为止报道的大多数生物传感器都是在实验室条件下制备、研究和使用的。大量生物传感器的可行性似乎已得到证明，而且它们的特性往往也已确定符合现代分析的要求。据作者称，生物传感器的操作稳定性几乎总是可以接受的。然而，对于商业化产品所必需的、能保持分析质量的长期储存，却很少有人研究。生物传感器的稳定性不仅必须在制造步骤或后续使用过程中得以维持，而且还必须在传感器从生产商到最终用户，经过批发商和/或零售商的整个商业保质期内保持稳定。我们大规模开发了用氧化还原酶等酶修饰的可再生表面电极的制造工艺。文中描述了该工艺的几个步骤，并研究了所得生物传感器的分析行为，并将其与制造过程中施加的不同限制条件的影响相关联。
