淋巴细胞膜同种抗原和免疫球蛋白的差异放射性标记：乳过氧化物酶催化细胞表面放射性碘化过程中过氧化氢浓度的变化

Differential radiolabelling of lymphocyte membrane alloantigens and immunoglobulins: variation of H2O2 concentration during lactoperoxidase catalyzed cell surface radio-iodination.

作者信息

Emerson S G, Reilly P, Cone R E

出版信息

J Immunogenet. 1979 Apr;6(2):87-97. doi: 10.1111/j.1744-313x.1979.tb00334.x.

DOI:10.1111/j.1744-313x.1979.tb00334.x

Abstract

The effect of H2O2 concentration on lactoperoxidase catalyzed cell surface radio-iodination and subsequent isolation of Murine splenic lymphocyte Ia, H-2K and Lyb-3 surface antigens and membrane immunoglobulins was studied. For most membrane polypeptides analyzed 0.3 mM H2O2 proved to be optimal for the recovery of radiolabelled antigens from detergent lysates of labelled cells by immunoprecipitation. Marked variations among surface antigens and membrane immunoglobulin polypeptide chains were observed for the iodination and recovery of these proteins above and below the optimal peroxide concentration. The results suggest that cell surface radio-iodination conditions should be standardized to the requirements of the particular membrane protein being studied. The differential iodination and recovery of discrete membrane components above and below optimal conditions may prove useful in the analysis of surface membrane protein structure and membrane association.

摘要

研究了过氧化氢（H₂O₂）浓度对乳过氧化物酶催化的细胞表面放射性碘化以及随后对小鼠脾淋巴细胞Ia、H-2K和Lyb-3表面抗原及膜免疫球蛋白的分离的影响。对于大多数分析的膜多肽，0.3 mM H₂O₂被证明是通过免疫沉淀从标记细胞的去污剂裂解物中回收放射性标记抗原的最佳浓度。在最佳过氧化物浓度之上和之下，这些蛋白质的碘化和回收在表面抗原和膜免疫球蛋白多肽链之间观察到明显差异。结果表明，细胞表面放射性碘化条件应根据所研究的特定膜蛋白的要求进行标准化。在最佳条件之上和之下离散膜成分的差异碘化和回收可能在表面膜蛋白结构和膜结合分析中证明是有用的。