叶酸受体-α是马尔堡病毒和埃博拉病毒进入细胞的辅助因子。

Folate receptor-alpha is a cofactor for cellular entry by Marburg and Ebola viruses.

作者信息

Chan S Y, Empig C J, Welte F J, Speck R F, Schmaljohn A, Kreisberg J F, Goldsmith M A

机构信息

Gladstone Institute of Virology and Immunology, San Francisco, CA 94141, USA.

出版信息

Cell. 2001 Jul 13;106(1):117-26. doi: 10.1016/s0092-8674(01)00418-4.

DOI:10.1016/s0092-8674(01)00418-4

PMID:11461707

Abstract

Human infections by Marburg (MBG) and Ebola (EBO) viruses result in lethal hemorrhagic fever. To identify cellular entry factors employed by MBG virus, noninfectible cells transduced with an expression library were challenged with a selectable pseudotype virus packaged by MBG glycoproteins (GP). A cDNA encoding the folate receptor-alpha (FR-alpha) was recovered from cells exhibiting reconstitution of viral entry. A FR-alpha cDNA was recovered in a similar strategy employing EBO pseudotypes. FR-alpha expression in Jurkat cells facilitated MBG or EBO entry, and FR-blocking reagents inhibited infection by MBG or EBO. Finally, FR-alpha bound cells expressing MBG or EBO GP and mediated syncytia formation triggered by MBG GP. Thus, FR-alpha is a significant cofactor for cellular entry for MBG and EBO viruses.

摘要

马尔堡病毒（MBG）和埃博拉病毒（EBO）感染人类会导致致命的出血热。为了确定MBG病毒所利用的细胞进入因子，用表达文库转导的不可感染细胞受到由MBG糖蛋白（GP）包装的可选择假型病毒的攻击。从表现出病毒进入重建的细胞中回收了编码叶酸受体α（FR-α）的cDNA。采用类似策略，利用EBO假型也回收了FR-α cDNA。Jurkat细胞中FR-α的表达促进了MBG或EBO的进入，而FR阻断试剂抑制了MBG或EBO的感染。最后，FR-α与表达MBG或EBO GP的细胞结合，并介导由MBG GP触发的合胞体形成。因此，FR-α是MBG和EBO病毒进入细胞的重要辅助因子。