假型病毒用于马尔堡病毒和埃博拉病毒。
Pseudotyped Viruses for Marburgvirus and Ebolavirus.
机构信息
Division of HIV/AIDS and Sex-transmitted Virus Vaccines, National Institutes for Food and Drug Control (NIFDC) and WHO Collaborating Center for Standardization and Evaluation of Biologicals, Beijing, China.
Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing, China.
出版信息
Adv Exp Med Biol. 2023;1407:105-132. doi: 10.1007/978-981-99-0113-5_6.
Marburg virus (MARV) and Ebola virus (EBOV) of the Filoviridae family are the most lethal viruses in terms of mortality rate. However, the development of antiviral treatment is hampered by the requirement for biosafety level-4 (BSL-4) containment. The establishment of BSL-2 pseudotyped viruses can provide important tools for the study of filoviruses. This chapter summarizes general information on the filoviruses and then focuses on the construction of replication-deficient pseudotyped MARV and EBOV (e.g., lentivirus system and vesicular stomatitis virus system). It also details the potential applications of the pseudotyped viruses, including neutralization antibody detection, the study of infection mechanisms, the evaluation of antibody-dependent enhancement, virus entry inhibitor screening, and glycoprotein mutation analysis.
马尔堡病毒（MARV）和埃博拉病毒（EBOV）属于丝状病毒科，就死亡率而言，它们是最致命的病毒。然而，由于需要生物安全四级（BSL-4）防护，抗病毒治疗的发展受到了阻碍。BSL-2 假型病毒的建立可为丝状病毒的研究提供重要工具。本章概述了丝状病毒的一般信息，然后重点介绍了复制缺陷型假型 MARV 和 EBOV 的构建（例如，慢病毒系统和水疱性口炎病毒系统）。它还详细介绍了假型病毒的潜在应用，包括中和抗体检测、感染机制研究、抗体依赖性增强作用评估、病毒进入抑制剂筛选和糖蛋白突变分析。
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