Effects of rejuvenation of blood on 2,3-diphosphoglycerate and adenosine triphosphate and mechanical resistance of red blood cells.

Blood collected in ACD was supplemented with PIGP solution or PIGPA solution. The presence of adenine enhanced the mechanical resistance to trauma of erythrocytes. The levels of 2,3-diphosphoglycerate and adenosine triphosphate were restored to a normal to subnormal level. The PIGPA media seemed satisfactory for rejuvenation of stored blood for freezing purposes.