Brecher M E, Zylstra-Halling V W, Pineda A A
Section of Transfusion Medicine, Mayo Clinic, Rochester, Minnesota 55905.
Am J Clin Pathol. 1991 Dec;96(6):767-9. doi: 10.1093/ajcp/96.6.767.
Rejuvenation of erythrocytes (biochemical modification of adenosine triphosphate and 2,3-diphosphoglycerate) preserved with currently available nutrient additive solutions has not been licensed or adequately studied. The ability of the commercially available solution PIPA to modify erythrocytes preserved with AS-1 or AS-3 for 35 and 42 days was studied. Use of PIPA produced greater-than-normal levels of adenosine triphosphate in the preserved erythrocytes. Restoration of 2,3-diphosphoglycerate was suboptimal at both periods. (PIPA treatment produces normal levels of 2,3-diphosphoglycerate in erythrocytes preserved for 35 days with citrate-phosphate-dextrose-adenine-1.) In erythrocytes preserved with AS-1 or AS-3 for 42 days, 2,3-diphosphoglycerate returned to normal levels after a second PIPA rejuvenation. This study demonstrates that biochemical modification is possible in erythrocytes preserved with AS-1 or AS-3 for 42 days.
使用目前可用的营养添加剂溶液保存的红细胞复苏（三磷酸腺苷和2,3 - 二磷酸甘油酸的生化修饰）尚未获得许可或得到充分研究。研究了市售溶液PIPA对用AS - 1或AS - 3保存35天和42天的红细胞进行修饰的能力。使用PIPA可使保存的红细胞中三磷酸腺苷水平高于正常水平。在这两个时间段内，2,3 - 二磷酸甘油酸的恢复都不理想。（PIPA处理可使用枸橼酸盐 - 磷酸盐 - 葡萄糖 - 腺嘌呤 - 1保存35天的红细胞中2,3 - 二磷酸甘油酸水平恢复正常。）在用AS - 1或AS - 3保存42天的红细胞中，第二次PIPA复苏后2,3 - 二磷酸甘油酸恢复到正常水平。这项研究表明，用AS - 1或AS - 3保存42天的红细胞进行生化修饰是可行的。
