Babitch J A, Blomstrand C, Hamberger A
Acta Neurol Scand. 1975 Mar;51(3):211-24. doi: 10.1111/j.1600-0404.1975.tb07602.x.
The in vitro protein synthetic capactiy of brain slices from guinea-pigs in the late stage (17-18 days post-induction) of experimental allergic encephalomyelitis was increased over that of Freund's adjuvant injected controls, as determined by the rate of (14C)-leucine incorporation into both tris-soluble and tris-insoluble proteins. All subcellular fractions prepared from incubated slices showed increased incorporation, with a crude nuclear fraction having the largest increase. Isolated brain mitochondria from EAE animals incorporated more amino acid into protein during the late stage of the disease, while isolated microsomes and "pH5 enzymes" show decreased amino acid incorporation compared with controls in the late stage of EAE. Polyacrylamide gel electrophoresis of acidic, soluble proteins isolated from (3H)-leucine labeled nuclear or synaptosomal fractions revealed that increases of incorporation were generalized, and not restricted to a few proteins.
通过将（14C）-亮氨酸掺入三羟甲基氨基甲烷可溶性和三羟甲基氨基甲烷不溶性蛋白质的速率测定，发现实验性变应性脑脊髓炎晚期（诱导后17 - 18天）豚鼠脑片的体外蛋白质合成能力比注射弗氏佐剂的对照增强。从孵育切片制备的所有亚细胞组分均显示掺入增加，其中粗核组分增加最大。来自实验性变应性脑脊髓炎动物的分离脑线粒体在疾病晚期将更多氨基酸掺入蛋白质中，而在实验性变应性脑脊髓炎晚期，分离的微粒体和“pH5酶”与对照相比显示氨基酸掺入减少。从（3H）-亮氨酸标记的核或突触体组分中分离的酸性可溶性蛋白质的聚丙烯酰胺凝胶电泳显示，掺入增加是普遍的，并不局限于少数蛋白质。
