Babitch J A, Blomstrand C, Hamberger A
Brain Res. 1975 Mar 28;86(3):459-67. doi: 10.1016/0006-8993(75)90896-3.
After in vitro incubation of brain slices from guinea pigs in the intermediate (10 days postinduction) stage of experimental allergic encephalomyelitis (EAE), incorporation of [14C]leucine into protein was increased in a glial-enriched fraction. By the late (17-18 days postinduction) stage of the disease, when EAE symptoms were manifest, both the neuronal- and glial-enriched fractions showed increased specific activity of their total protein. SDS polyacrylamide gel electrophoresis showed that the increased leucine incorporation occurred in those proteins which labeled in a control material. After intraperitoneal injection of [3H]leucine the incorporated radioactivity was slightly increased in unfractionated brains from EAE animals. The neuronal-glial ratios for protein-bound radioactivity indicated that the increased incorporation resided mainly in the glial population.
在实验性变态反应性脑脊髓炎（EAE）中间阶段（诱导后10天），将豚鼠脑片进行体外孵育后，富含胶质细胞的组分中[14C]亮氨酸掺入蛋白质的量增加。在疾病后期（诱导后17 - 18天），当EAE症状明显时，富含神经元和富含胶质细胞的组分中其总蛋白的比活性均增加。十二烷基硫酸钠聚丙烯酰胺凝胶电泳显示，亮氨酸掺入增加发生在对照材料中标记的那些蛋白质中。腹腔注射[3H]亮氨酸后，EAE动物未分级的脑中掺入的放射性略有增加。蛋白质结合放射性的神经元 - 胶质细胞比率表明，掺入增加主要存在于胶质细胞群体中。
