Cook N B, Ward W R, Dobson H
University of Wisconsin-Madison, School of Veterinary Medicine, 53706, USA.
Vet Rec. 2001 Jun 23;148(25):769-72. doi: 10.1136/vr.148.25.769.
Samples of blood and milk were taken from clinically ketotic cows at 12-hour intervals before and after treatment The concentration of beta-hydroxybutyrate was determined in plasma and milk, and the concentration of acetone was determined in milk. Measurement of milk acetone had the optimal combination of sensitivity, specificity and positive predictive value to identify ketotic cows and was subsequently used in a comparison of the fertility of ketotic and normal cows. Two samples of milk were taken approximately two weeks apart, between 12 and 60 days after calving, from 410 cows on three farms and the concentration of acetone in the milk was measured. Thirty cows with milk acetone concentrations of at least 0.4 mmol/litre were compared with paired control cows with milk acetone concentrations less than 0.3 mmol/litre. The ketotic cows had a significantly longer calving-to-conception interval and a significantly higher culling rate due to a failure to conceive (P<0.05).
在治疗前后，每隔12小时从临床患酮血症的奶牛身上采集血液和牛奶样本。测定血浆和牛奶中β-羟基丁酸的浓度，并测定牛奶中丙酮的浓度。牛奶丙酮测定在识别患酮血症奶牛方面具有敏感性、特异性和阳性预测值的最佳组合，随后用于比较患酮血症奶牛和正常奶牛的繁殖力。在三个农场的410头奶牛产犊后12至60天内，每隔约两周采集两份牛奶样本，并测定牛奶中丙酮的浓度。将30头牛奶丙酮浓度至少为0.4 mmol/升的奶牛与配对的牛奶丙酮浓度低于0.3 mmol/升的对照奶牛进行比较。患酮血症的奶牛产犊至受孕间隔明显更长，且因未能受孕而被淘汰的比例明显更高（P<0.05）。
