Ježek J, Cincović M R, Nemec M, Belić B, Djoković R, Klinkon M, Starič J
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Pol J Vet Sci. 2017 Sep 26;20(3):507-512. doi: 10.1515/pjvs-2017-0061.
Ketosis is a very frequent metabolic disease in dairy cows, resulting in lower milk production, impaired fertility and increased frequency of other diseases. The course of the disease is often subclinical, so early detection is very important. The aim of the study was to investigate the relation between the concentration of beta-hydroxybutyrate in blood and milk and to determine the cut-off value in milk for detection of subclinical ketosis. The study included 94 cows, which were in the first third of lactation. Beta-hydroxybutyrate (BHB) concentrations were measured in blood and milk serum using a biochemical analyser. The average concentration of BHB in the blood serum samples was 1.14 mmol/L while in the milk it was about ten times lower at 0.117 mmol/L. A statistically significant positive correlation between the concentration of BHB in blood and milk (r=0.705, p<0.001) was found. In cows with BHB in blood below 2.0 mmol/L a stronger correlation between blood and milk BHB was established (r=0.658, p<0.001) than in cows with blood BHB above 2.0 mmol/L (r=-0.292, p=0.206). Therefore, BHB in milk is a very suitable indicator in the diagnosis of subclinical ketosis as there is a good correlation between BHB in the blood and milk of cows with subclinical ketosis. The cut-off concentration of BHB in milk set at ≥0.080 mmol/L (AUC=0.91±0.03; p<0.001) is a significant indicator for subclinical ketosis in dairy cows. The sensitivity of the test was 94% and specificity 74%. Beta-hydroxybutyrate in milk is a good indicator of subclinical ketosis in dairy cows and can be measured accurately with a biochemical analyser.
酮病是奶牛中一种非常常见的代谢性疾病，会导致产奶量降低、繁殖力受损以及其他疾病发病率增加。该病病程通常为亚临床型，因此早期检测非常重要。本研究的目的是调查血液和牛奶中β-羟基丁酸酯浓度之间的关系，并确定用于检测亚临床酮病的牛奶临界值。该研究纳入了94头处于泌乳前三分之一阶段的奶牛。使用生化分析仪测量血液和牛奶血清中的β-羟基丁酸酯（BHB）浓度。血清样本中BHB的平均浓度为1.14 mmol/L，而牛奶中的浓度约低十倍，为0.117 mmol/L。发现血液和牛奶中BHB浓度之间存在统计学上显著的正相关（r = 0.705，p < 0.001）。血液中BHB低于2.0 mmol/L的奶牛，其血液和牛奶中BHB的相关性（r = 0.658，p < 0.001）比血液中BHB高于2.0 mmol/L的奶牛（r = -0.292，p = 0.206）更强。因此，牛奶中的BHB是诊断亚临床酮病的非常合适的指标，因为亚临床酮病奶牛的血液和牛奶中的BHB之间存在良好的相关性。牛奶中BHB的临界浓度设定为≥0.080 mmol/L（AUC = 0.91±0.03；p < 0.oo1）是奶牛亚临床酮病的显著指标。该检测的敏感性为94%，特异性为74%。牛奶中的β-羟基丁酸酯是奶牛亚临床酮病的良好指标，并且可以使用生化分析仪准确测量。
