来自酿酒酵母的脱氧尿苷三磷酸核苷酸水解酶的结晶及初步X射线晶体学分析。
Crystallization and preliminary X-ray crystallographic analysis of deoxyuridine triphosphate nucleotidohydrolase from Saccharomyces cerevisiae.
作者信息
Han B W, Lee J Y, Yang J K, Lee B I, Suh S W
机构信息
School of Chemistry and Molecular Engineering, College of Natural Sciences, Seoul National University, Seoul 151-742, South Korea.
出版信息
Acta Crystallogr D Biol Crystallogr. 2001 Aug;57(Pt 8):1147-9. doi: 10.1107/s0907444901007909. Epub 2001 Jul 23.
Deoxyuridine triphosphate nucleotidohydrolase (dUTPase) from Saccharomyces cerevisiae is essential for cell viability. It has been overexpressed in Escherichia coli and has been crystallized at 296 K using polyethylene glycol (PEG) 1500 as a precipitant. The crystals belong to the orthorhombic space group P2(1)2(1)2(1), with unit-cell parameters a = 59.48, b = 138.54, c = 157.91 A, alpha = beta = gamma = 90 degrees. Two molecules of trimeric dUTPase from S. cerevisiae are present in the asymmetric unit, giving a crystal volume per protein mass (V(M)) of 3.36 A(3) Da(-1) and a solvent content of 63%. The diffraction limit of the crystals could be significantly extended by the crystal-annealing procedure. A set of native data extending to 2.7 A resolution has been collected at 100 K using synchrotron X-rays.
来自酿酒酵母的脱氧尿苷三磷酸核苷酸水解酶（dUTPase）对细胞活力至关重要。它已在大肠杆菌中过表达，并使用聚乙二醇（PEG）1500作为沉淀剂在296 K下结晶。晶体属于正交晶系空间群P2(1)2(1)2(1)，晶胞参数为a = 59.48，b = 138.54，c = 157.91 Å，α = β = γ = 90°。不对称单元中存在两个来自酿酒酵母的三聚体dUTPase分子，蛋白质质量的晶体体积（V(M)）为3.36 ų Da⁻¹，溶剂含量为63%。通过晶体退火程序可显著扩展晶体的衍射极限。已在100 K下使用同步加速器X射线收集了一套分辨率达2.7 Å的天然数据。
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