Bajaj Mamta, Moriyama Hideaki
School of Biological Sciences, University of Nebraska-Lincoln, Manter Hall, Lincoln, Nebraska 68588-0304, USA.
Acta Crystallogr Sect F Struct Biol Cryst Commun. 2007 May 1;63(Pt 5):409-11. doi: 10.1107/S1744309107016004. Epub 2007 Apr 14.
The deoxyuridine triphosphate nucleotidohydrolase gene from Arabidopsis thaliana was expressed and the gene product was purified. Crystallization was performed by the hanging-drop vapour-diffusion method at 298 K using 2 M ammonium sulfate as the precipitant. X-ray diffraction data were collected to 2.2 A resolution using Cu K alpha radiation. The crystal belongs to the orthorhombic space group P2(1)2(1)2(1), with unit-cell parameters a = 69.90, b = 70.86 A, c = 75.55 A. Assuming the presence of a trimer in the asymmetric unit, the solvent content was 30%, with a V(M) of 1.8 A3 Da(-1).
对拟南芥的脱氧尿苷三磷酸核苷酸水解酶基因进行了表达，并对基因产物进行了纯化。采用悬滴气相扩散法，以2 M硫酸铵作为沉淀剂，在298 K下进行结晶。使用Cu Kα辐射收集了分辨率为2.2 Å的X射线衍射数据。晶体属于正交晶系空间群P2(1)2(1)2(1)，晶胞参数a = 69.90、b = 70.86 Å、c = 75.55 Å。假设不对称单元中存在三聚体，溶剂含量为30%，V(M)为1.8 Å3 Da(-1)。
