Yamada Y, Saijo S, Sato T, Igarashi N, Usui H, Fujiwara T, Tanaka N
Department of Life Science, Graduate School of Bioscience and Biotechnology, Tokyo Institute of Technology, 4259 Nagatsuta, Midori-ku, Yokohama 226-8501, Japan.
Acta Crystallogr D Biol Crystallogr. 2001 Aug;57(Pt 8):1157-8. doi: 10.1107/s0907444901008927. Epub 2001 Jul 23.
Catalase-peroxidases are bifunctional enzymes found in many microorganisms. Crystals of catalase-peroxidase from the halophilic archaeon Haloarcula marismortui were obtained using the hanging-drop vapour-diffusion method. The rhombic plate-shaped crystals were grown from purified protein solution using (NH(4))(2)SO(4) as precipitant at 293 K. The crystal belongs to the monoclinic system, space group C2, and diffracted beyond 2.0 A resolution.
过氧化氢酶是在许多微生物中发现的双功能酶。使用悬滴气相扩散法获得了嗜盐古菌盐沼盐杆菌过氧化氢酶的晶体。菱形板状晶体是在293K下，以硫酸铵为沉淀剂，从纯化的蛋白质溶液中生长出来的。该晶体属于单斜晶系，空间群为C2，衍射分辨率超过2.0埃。
