Schinkel H, Hertzberg M, Wingsle G
Department of Forest Genetics and Plant Physiology, Swedish University of Agricultural Sciences, Umeå.
Planta. 2001 Jun;213(2):272-9. doi: 10.1007/s004250000505.
Several CuZn-superoxide dismutases (SODs; EC 1.15.1.1) were cloned from hybrid aspen (Populus tremula L. x tremuloides Michx.). Two of the cloned genes encode representatives of a novel type of CuZn-SOD and we named it HipI-SOD because of its high isoelectric point (> or =9). The SODs were cloned by screening a cDNA library with a probe based on a Scots pine (Pinus sylvestris L.) CuZn-SOD that is predominantly located extracellularly. The expression pattern of HipI-SOD was examined using a Northern blot technique and compared with the expression patterns of cytosolic and chloroplastic SODs. Distinct expression patterns were observed for the three types of CuZn-SOD, with HipI-SODs showing strong expression in apical tissues. Southern blots as well as protein analysis suggest that these novel HipI-SODs belong to a small gene family, one member of which might be monomeric.
从杂种山杨（Populus tremula L. x tremuloides Michx.）中克隆出了几种铜锌超氧化物歧化酶（SODs；EC 1.15.1.1）。其中两个克隆基因编码一种新型铜锌超氧化物歧化酶的代表，由于其高的等电点（≥9），我们将其命名为HipI-SOD。通过用基于主要位于细胞外的欧洲赤松（Pinus sylvestris L.）铜锌超氧化物歧化酶的探针筛选cDNA文库来克隆这些超氧化物歧化酶。使用Northern印迹技术检测了HipI-SOD的表达模式，并与胞质和叶绿体超氧化物歧化酶的表达模式进行了比较。观察到三种类型的铜锌超氧化物歧化酶有不同的表达模式,其中HipI-SOD在顶端组织中表现出强烈表达。Southern印迹以及蛋白质分析表明，这些新型HipI-SOD属于一个小基因家族，其中一个成员可能是单体。
