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嗜热栖热菌HB8的5S核糖体RNA单晶及初步X射线衍射数据。

Single crystals of 5S rRNA from Thermus thermophilus HB8 and preliminary X-ray diffraction data.

作者信息

Morikawa K, Kawakami M, Takemura S

出版信息

Nucleic Acids Symp Ser. 1982(11):173-6.

PMID:7183958
Abstract

5S rRNA from extremely thermophilic bacteria, Thermus thermophilus HB8, was crystallized. Using a vapour diffusion method, single crystals were grown with sufficient sizes for x-ray diffraction study. The crystals belong to space group P3(1)21 (or enatiomorph P3(2)21) with a = 99 A, b = 99 A and c = 360 A. A unit cell contains probably 36 molecules corresponding to 6 molecules/asymmetric unit. A smaller pseudo-lattice seems to be constructed with the same lengths for a- and b-axis, and 120 A for c-axis. Reflections were observed up to 25 A on x-ray photographs.

摘要

来自嗜热栖热菌HB8的5S核糖体RNA被结晶出来。采用气相扩散法,生长出了尺寸足够大的用于X射线衍射研究的单晶。这些晶体属于空间群P3(1)21(或对映体P3(2)21),a = 99埃,b = 99埃,c = 360埃。一个晶胞可能包含36个分子,相当于6个分子/不对称单位。似乎构建了一个较小的假晶格,其a轴和b轴长度相同,c轴长度为120埃。在X射线照片上观察到了高达25埃的反射。

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Nucleic Acids Symp Ser. 1982(11):173-6.
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