Ragan M A
Institute for Molecular Bioscience, The University of Queensland, Brisbane, Qld 4072, Australia.
FEMS Microbiol Lett. 2001 Jul 24;201(2):187-91. doi: 10.1111/j.1574-6968.2001.tb10755.x.
Surrogate methods for detecting lateral gene transfer are those that do not require inference of phylogenetic trees. Herein I apply four such methods to identify open reading frames (ORFs) in the genome of Escherichia coli K12 that may have arisen by lateral gene transfer. Only two of these methods detect the same ORFs more frequently than expected by chance, whereas several intersections contain many fewer ORFs than expected. Each of the four methods detects a different non-random set of ORFs. The methods may detect lateral ORFs of different relative ages; testing this hypothesis will require rigorous inference of trees.
用于检测横向基因转移的替代方法是那些不需要推断系统发育树的方法。在此，我应用四种这样的方法来鉴定大肠杆菌K12基因组中可能通过横向基因转移产生的开放阅读框（ORF）。这些方法中只有两种比随机预期更频繁地检测到相同的ORF，而几个交叉点包含的ORF比预期少得多。这四种方法中的每一种都检测到一组不同的非随机ORF。这些方法可能检测到不同相对年龄的横向ORF；检验这一假设将需要对树进行严格的推断。
