Fujino T, Takei Y A, Sone H, Ioka R X, Kamataki A, Magoori K, Takahashi S, Sakai J, Yamamoto T T
Tohoku University Gene Research Center, Sendai 981-8555, Japan.
J Biol Chem. 2001 Sep 21;276(38):35961-6. doi: 10.1074/jbc.M106651200. Epub 2001 Jul 24.
In this study, we identified and characterized two murine cDNAs encoding medium-chain acyl-CoA synthetase (MACS). One, designated MACS1, is a novel protein and the other the product of the Sa gene (Sa protein), which is preferentially expressed in spontaneously hypertensive rats. Based on the murine MACS1 sequence, we also identified the location and organization of the human MACS1 gene, showing that the human MACS1 and Sa genes are located in the opposite transcriptional direction within a 150-kilobase region on chromosome 16p13.1. Murine MACS1 and Sa protein were overexpressed in COS cells, purified to homogeneity, and characterized. Among C4-C16 fatty acids, MACS1 preferentially utilizes octanoate, whereas isobutyrate is the most preferred fatty acid among C2-C6 fatty acids for Sa protein. Like Sa gene transcript, MACS1 mRNA was detected mainly in the liver and kidney. Subcellular fractionation revealed that both MACS1 and Sa protein are localized in the mitochondrial matrix. (14)C-Fatty acid incorporation studies indicated that acyl-CoAs produced by MACS1 and Sa protein are utilized mainly for oxidation.
在本研究中，我们鉴定并表征了两个编码中链酰基辅酶A合成酶（MACS）的小鼠cDNA。其中一个命名为MACS1，是一种新蛋白，另一个是Sa基因的产物（Sa蛋白），它在自发性高血压大鼠中优先表达。基于小鼠MACS1序列，我们还确定了人类MACS1基因的位置和结构，表明人类MACS1和Sa基因位于16号染色体p13.1上150千碱基区域内相反的转录方向。小鼠MACS1和Sa蛋白在COS细胞中过表达，纯化至同质，并进行表征。在C4 - C16脂肪酸中，MACS1优先利用辛酸，而对于Sa蛋白来说，异丁酸是C2 - C6脂肪酸中最优先利用的脂肪酸。与Sa基因转录本一样，MACS1 mRNA主要在肝脏和肾脏中检测到。亚细胞分级分离显示MACS1和Sa蛋白都定位于线粒体基质中。（14）C - 脂肪酸掺入研究表明，MACS1和Sa蛋白产生的酰基辅酶A主要用于氧化。
