胸苷激酶在宫颈癌患者中的临床价值。

Clinical value of thymidine kinase in patients with cervical carcinoma.

作者信息

Fujiwaki R, Hata K, Moriyama M, Iwanari O, Katabuchi H, Okamura H, Miyazaki K

机构信息

Department of Obstetrics and Gynecology, Shimane Medical University, Izumo, Japan.

出版信息

Oncology. 2001;61(1):47-54. doi: 10.1159/000055352.

DOI:10.1159/000055352

PMID:11474248

Abstract

OBJECTIVE

Our purpose was to determine the clinical value of thymidine kinase (TK), which is an important pyrimidine pathway enzyme involved in salvage DNA synthesis, in patients with cervical carcinoma.

METHODS

We examined TK mRNA expression by reverse transcription polymerase chain reaction in 19 tissue specimens of invasive cervical carcinoma and 9 normal cervices and related it to thymidylate synthase (TS) and thymidine phosphorylase (TP) mRNA expressions. Serum TK level was determined by radioenzymatic assay in 79 patients with invasive cervical carcinoma, 7 patients with microinvasive carcinoma, 21 patients with carcinoma in situ and 32 normal women.

RESULTS

TK mRNA expression was upregulated in invasive cervical carcinoma compared with the normal cervix (p < 0.05) and significantly correlated with TS mRNA expression (p < 0.0001) but not with TP mRNA expression. The serum TK level was significantly higher in patients with invasive carcinoma than in normal women and patients with carcinoma in situ (p < 0.01 and p < 0.05). In patients with invasive cervical carcinoma, the serum TK level significantly correlated with TK mRNA expression (p < 0.05), but not with any conventional clinicopathologic factors. High serum TK levels significantly correlated with a poorer survival (p < 0.05), and multivariate analysis showed serum TK level to be an independent prognostic factor (p < 0.05).

CONCLUSION

TK may play an important role in influencing the malignant behavior of cervical carcinoma, and measurement of the serum TK level may be useful in predicting survival in patients with cervical carcinoma.

摘要

目的

我们的目的是确定胸苷激酶（TK）在宫颈癌患者中的临床价值，胸苷激酶是参与DNA补救合成的一种重要嘧啶途径酶。

方法

我们通过逆转录聚合酶链反应检测了19例浸润性宫颈癌组织标本和9例正常宫颈组织中TK mRNA的表达，并将其与胸苷酸合成酶（TS）和胸苷磷酸化酶（TP）mRNA的表达进行关联分析。采用放射酶法测定了79例浸润性宫颈癌患者、7例微浸润癌患者、21例原位癌患者和32例正常女性的血清TK水平。

结果

与正常宫颈相比，浸润性宫颈癌中TK mRNA表达上调（p < 0.05），且与TS mRNA表达显著相关（p < 0.0001），但与TP mRNA表达无关。浸润性癌患者的血清TK水平显著高于正常女性和原位癌患者（p < 0.01和p < 0.05）。在浸润性宫颈癌患者中，血清TK水平与TK mRNA表达显著相关（p < 0.05），但与任何传统临床病理因素均无关。高血清TK水平与较差的生存率显著相关（p < 0.05），多因素分析显示血清TK水平是一个独立的预后因素（p < 0.05）。