Schunk M, Jelinek T, Wetzel K, Nothdurft H D
Department of Infectious Diseases and Tropical Medicine, University of Munich, Germany.
Eur J Clin Microbiol Infect Dis. 2001 Jun;20(6):389-91. doi: 10.1007/pl00011279.
Two commercially produced enzyme immunoassays (EIAs) to detect antigens of Giardia lamblia and Entamoeba histolytica in stool specimens were evaluated. A total of 276 stool specimens were collected from patients who presented with various medical complaints in the outpatient clinic of the Department of Infectious Diseases and Tropical Medicine, University of Munich. Every specimen was examined by conventional microscopy and tested by both EIA kits. When microscopy was used as the reference standard, the EIA kit detecting Giardia lamblia showed a sensitivity of 100% and a specificity of 99.6%. The EIA kit detecting Entamoeba histolytica had a sensitivity of 81.8% and a specificity of 99.2%. Both tests showed no cross-reactivity with other intestinal protozoa. Antigen detection by EIA has the potential to become a valuable tool capable of making stool diagnostics more effective, although it should not be considered as a replacement for microscopic examination, since other potential pathogens could otherwise escape detection.
对两种用于检测粪便标本中蓝氏贾第鞭毛虫和溶组织内阿米巴抗原的商业化酶免疫测定法（EIA）进行了评估。从慕尼黑大学传染病与热带医学系门诊有各种医学主诉的患者中总共收集了276份粪便标本。每份标本均通过传统显微镜检查，并使用两种EIA试剂盒进行检测。当以显微镜检查作为参考标准时，检测蓝氏贾第鞭毛虫的EIA试剂盒灵敏度为100%，特异性为99.6%。检测溶组织内阿米巴的EIA试剂盒灵敏度为81.8%，特异性为99.2%。两种检测均未显示与其他肠道原生动物有交叉反应。通过EIA进行抗原检测有可能成为一种有价值的工具，使粪便诊断更有效，尽管不应将其视为显微镜检查的替代方法，因为否则其他潜在病原体可能会漏检。
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